Establishment of Acquired Aplastic Anemia SD Rat Model.
10.19746/j.cnki.issn.1009-2137.2020.03.034
- Author:
Ping-Ping WANG
1
;
Jia-Ni LI
1
;
Xin-Long SONG
2
;
Ai-Di WANG
1
;
Bao-Shan LIU
3
,
4
Author Information
1. Department of Traditional Chinese Medicine, Tianjin Medical University General Hospital, Tianjin 300052, China.
2. Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
3. Department of Traditional Chinese Medicine, Tianjin Medical University General Hospital, Tianjin 300052, China,E-mail: lbs-cd@
4. com.
- Publication Type:Journal Article
- MeSH:
Anemia, Aplastic;
Animals;
Bone Marrow;
Bone Marrow Cells;
Cyclophosphamide;
Rats;
Rats, Sprague-Dawley
- From:
Journal of Experimental Hematology
2020;28(3):927-931
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a stable and rapidly rat model acquired aplastic anemia.
METHODS:The SD rats were exposed to Csγ-ray at 3.5 and 4.0 Gy ( 91 cGy/min), and intraperitoneally injected with CTX at 35 mg/( kg·d) and CHL at 45 mg/( kg·d) in d 4, 6 and 8 after irradiation; the WBC, platelet and reticulocyte counts in peripheral blood, the smears and nucleated cells counts of bone marrow were observed.
RESULTS:The levels of peripheral blood 3-lineage cells of SD rats treated with Csγ-irradiation combined with cyclophosphamide and chloramphenicol were significantly reduced, among which white blood cells, platelets and reticulocytes decreased rapidly, and the number of bone marrow nucleated cells decreased significantly; bone marrow pathological sections showed severe reduction of hematopoietic cells, and the non-hematopoietic cells such as fat cells increased, and a serve or lightly reduction of bone marrow cells were found.
CONCLUSION:The rat model established by Csγ-ray irradiation combined with cyclophosphamide and chloramphenicol meets the clinical characteristics of aplastic anemia, and this study provides a stable rat model for the study of new therapeutic drugs for acquired aplastic anemia.
