Analysis of Genome-Wide DNA Methylation Differences in Umbilical Cord Blood Nucleated Red Blood Cells Between Term and Preterm Infants.
10.19746/j.cnki.issn.1009-2137.2020.03.037
- Author:
Nan-Nan YANG
1
;
Zhen-Yuan LUO
1
;
Man ZHOU
2
;
Hong LIN
3
;
Wei WANG
4
;
Sheng-Wen HUANG
5
Author Information
1. Department of Laboratorial Medicine, Guizhou Provincial People's Hospital, Guiyang 550002, Guizhou Province, China.
2. Department of Obstetrics, Guizhou Provincial People's Hospital, Guiyang 550002, Guizhou Province, China.
3. Department of Obstetrics, Guiyang Maternal and Child Health Hospital, Guiyang 550003, Guizhou Province, China.
4. Department of Obstetrics, The Second People's Hospital of Guiyang, Guiyang 550023, Guizhou Province, China.
5. Department of Laboratorial Medicine, Guizhou Provincial People's Hospital, Guiyang 550002, Guizhou Province, China,E-mail: hsw713@sina.com.
- Publication Type:Journal Article
- MeSH:
DNA;
DNA Methylation;
Epigenesis, Genetic;
Fetal Blood;
Genome, Human;
Humans;
Infant, Newborn;
Infant, Premature
- From:
Journal of Experimental Hematology
2020;28(3):942-947
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To analyze the genome-wide DNA methylation differences in umbilical cord blood nucleated red blood cells (NRBCs) between term and preterm infants by using the methylation gene chip technology, and to screen the genes of differential methylation and biological signaling pathways which may be related to the expression of γ-globin gene (HBG).
METHODS:Umbilical cord bloods of eight term infants and eight preterm infants were collected, and NRBCs of each sample was isolated, then genome DNA was extracted and bisulfite conversion was performed. The DNA methylation sites were detected by using the Illumina 850K BeadChip. Differential DNA methylation sites were screened, and the function of genes with differential methylation was analyzed by using GO and KEGG enrichment analysis.
RESULTS:Compared with the preterm group, 4749 differential DNA methylation sites of term group were screened out, including 4359 hypomethylation sites and 390 hypermethylation sites. GO and KEGG analysis indicated that the function of genes with differential methylation mainly involved in the hemopoietic system, growth and development process, Wnt and Notch signal pathways.
CONCLUSION:The differentical methylation sites at genome-wide level in umbilicar cord blood NRBC of term and preterm infants have been obtained, and the signal pathway and genes which possibily related with swiching the expression of γ-globin gene to β-globin gene have been screened-out. This study provide the new targets for studing the mechamism regulating expression of HBG gene.
