Identification of a novel KIR3DL3*064 allele by cDNA cloning and sequencing.
10.3760/cma.j.issn.1003-9406.2020.08.022
- VernacularTitle:cDNA分子克隆法鉴定
KIR3DL3*064新等位基因
- Author:
Dongmei NIE
1
;
Siqi CAI
;
Guobin ZHANG
;
Zhihui DENG
Author Information
1. Shenzhen Blood Center, Shenzhen, Guangdong 518035, China. zhihui_deng@sina.cn.
- Publication Type:Journal Article
- From:
Chinese Journal of Medical Genetics
2020;37(8):895-897
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To report on a novel KIR3DL3 allele identified in a southern Han Chinese individual.
METHODS:Peripheral blood sample was collected from a voluntary blood donor with inconclusive result by KIR3DL3 sequence-based typing (SBT). Total mRNA was extracted and subjected to reverse transcription to obtain KIR3DL3 cDNA, which was then amplified by PCR with a pair of KIR3DL3-specific primers. The product was subjected to cDNA cloning and sequencing.
RESULTS:cDNA cloning and sequencing have identified a wide-type KIR3DL3*00802 allele and a novel KIR3DL3*064 allele. The latter differed from KIR3DL3*00601 by a missense variant at codon 374[c.1184 C>T (p.Thr374Ile)] in exon 9. The novel KIR3DL3 allele has been officially assigned by the KIR subcommittee of World Health Organization Nomenclature Committee for factors of HLA system.
CONCLUSION:cDNA cloning and sequencing may be used to distinguish inconclusive results in KIR3DL3 SBT in order to identify novel KIR alleles.
