Genotypic drug resistance of HIV-infected MSM who failedin antiviral therapy in Yunnan Province
10.19485/j.cnki.issn2096-5087.2020.10.004
- VernacularTitle:云南省男男性行为人群HIV/AIDS病例抗病毒治疗失败基因型耐药分析
- Author:
RUAN Wei yueqing
1
;
LIU Jiafa
;
ZHANG Mi
;
LI Jianjian
;
YANG Bihui
;
DENG Xuemei
;
Dong Xingqi
Author Information
1. Kunming Medical University
- Publication Type:Journal Article
- Keywords:
men who have sex with men human immunodeficiency virus antiviral therapy genotype drug resistance
- From:
Journal of Preventive Medicine
2020;32(10):987-991
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To learn the genotypic drug resistance of men who have sex with men ( MSM ) with HIV who failed in antiviral therapy in Yunnan Province, in order to provide basis for improving the effect of antiviral therapy.
Methods:The patients who were infected with HIV-1, homosexual transmitted and failed in antiviral therapy in Yunnan Province from 2014 to 2019 were recruited. Their plasma samples were tested by reverse transcription nested polymerase chain reaction ( RT-nPCR ) , the fragments were spliced using ContigExpress, and the resistance to 8 protease inhibitors ( PIs ) , 7 nucleoside reverse transcriptase inhibitors ( NRTIs ) and 5 non-nucleoside reverse transcriptase inhibitors ( NNRTIs ) were obtained from the HIV drug resistance data website of Stanford University.
Results:A total of 205 HIV/AIDS cases were included, 169 positive plasma samples were amplified, 112 cases were drug resistant, and the rate of drug resistance was 66.27%. The patients who were aged 30-49 years ( 76.09% ) , had genotype of CRF01_AE ( 76.34% ) or treated by AZT+3TC+NVP ( 77.08% ) had higher resistance rate. The resistance rates of NNRTIs, NRTIs and PIs were 62.72%, 49.70% and 2.96%, respectively; the resistance rates of NVP and EFV in NNRTIs were 62.72% and 61.54%. The main mutation site associated with NNRTIs was K103, accounting for 21.89% ( 37 cases ) ; the main mutation site associated with NRTIs was M184, accounting for 39.64% ( 67 cases ) ; the main mutation sites associated with PIs were M46L/K, accounting for 2.96% ( 5 cases ) , resulting in high resistance to NFV.
Conclusions:The drug resistance rate of HIV-infected MSM with failure of antiviral therapy in Yunnan Province is relatively high, with CRF01_AE as the main gene subtype of drug resistance. The drug resistance rate of NNRTIs is relatively high, especially NVP and EFV.
- Full text:云南省男男性行为人群HIV_AIDS病例抗病毒治疗失败基因型耐药分析.pdf
