Clinical significance of deubiquitinase UCHL5 expression in thyroid carcinoma and its relationship with malignant behaviors of carcinoma cells
10.3872/j.issn.1007-385x.2020.06.013
- VernacularTitle:去泛素化酶UCHL5在甲状腺癌组织中表达的临床意义及其与肿瘤细胞 恶性行为的关系
- Author:
REN Li
1
;
MA Shan
2
;
FENG Chao
1
;
GU Yi
1
;
LIU Tong
1
;
ZHANG Bowei
1
;
CHEN Chao
1
;
SONG Zhe
1
Author Information
1. Department of Vascular and Thyroid Surgery, Sichuan Provincial People's Hospital, Sichuan Academy of Medical Sciences, Chengdu 610072, Sichuan, China
2. Department of Breast Thyroid Surgery, the Fifth People's Hospital of Chengdu, Chengdu 611130, Sichuan, China
- Publication Type:Clinical Trial
- Keywords:
thyroid carcinoma;
ubiquitin carboxyl-terminal hydrolase L5 (UCHL5);
KTC-1 cells;
WRO cells;
de ubiquitination;
malignant proliferation;
tumor metastasis
- From:
Chinese Journal of Cancer Biotherapy
2020;27(6):671-677
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective: To explore the clinical significance and in vitro biological effect of ubiquitin carboxyl-terminal hydrolase L5 (UCHL5) expression in thyroid carcinoma (TC) tissues. Methods: TCGAdata were used to analyze the expression of UCHL5 in thyroid carcinoma tissues and its relationship with the prognosis of patients. 82 pairs of TC tissues and corresponding adjacent tissues were collected in the Department of Vascular and Thyroid Surgery, Sichuan Provincial People's Hospital from May 2018 to July 2019; TC cell lines (KTC-1 and WRO) were cultured in vitro, and transfected with UCHL5 overexpression vectors or their control vectors via lentivirus. The mRNAand protein expressions of UCHL5 and B-Raf proto-oncogene serine/threonine-protein kinase (BRAF) in tissues and cells were detected by qPCR and Western blotting, respectively. Cell proliferation was detected by CCK-8, and cell invasion and migration were detected by Transwell and Wound-healing experiments. Results: The expression of UCHL5 was low in TC tissues (P<0.01), and its expression was upregulated in tumor tissues with high TNM stage (P<0.01). The expression of UCHL5 was significantly correlated with BRAF expression and TNM stage of patients (all P<0.01), but not significantly related with patient's age, gender, pathological type and BRAF mutation (all P>0.05). In vitro overexpression of UCHL5 in KTC-1 and WRO cells could significantly promote BRAF expression, cell proliferation and metastasis (all P<0.01). Conclusion: The expression of UCHL5 is low in TC tissue, but upregulated with tumor progression. The high expression of UCHL5 in TC patients suggests poor prognosis. Meanwhile, UCHL5 can promote the malignant behaviors of TC cells in vitro.
- Full text:20200613.pdf
