Chiral column for separation of steroidal saponin analogs in Asparagus cochinchinensis
10.16438/j.0513-4870.2020-0043
- VernacularTitle:手性色谱柱用于天冬中甾体皂苷类似物的分离
- Author:
Lin GAO
1
;
Bei WANG
2
;
Xu PANG
2
;
Meng-meng ZHANG
1
;
Jie ZHANG
2
;
Xiao-juan CHEN
2
;
Bai-ping MA
2
Author Information
1. Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China; Institute of Radiation Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China
2. Institute of Radiation Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China
- Publication Type:Research Article
- Keywords:
italic>Asparagus cochinchinensis;
steroidal saponin;
separation;
chiral chromatographic column
- From:
Acta Pharmaceutica Sinica
2020;55(6):1245-1250
- CountryChina
- Language:Chinese
-
Abstract:
italic>Asparagus cochinchinensis is a commonly used traditional Chinese medicine with steroidal saponins as its main active ingredients. Due to the structural similarity and size of the steroidal saponins, these compounds cannot always be effectively separated by a combination of normal phase silica gel column chromatography and reversed phase ODS column chromatography. In this experiment, chromatographic columns with different separation mechanisms were systematically screened, and it was found that a chiral chromatographic cellulose column could effectively separate these components. This column was used to separate 3 mixtures to obtain 6 single compounds (1-6). Structural identification showed that the singular structural difference between these poorly separated components resides in a terminal glycosyl group (xylose or rhamnose) in the C-3 glycosyl chain, and compounds 4 and 6 are two new steroidal saponins. Since the structures of compounds are often unknown during the isolation and purification of natural products, chiral columns are rarely used. This study suggests that chiral chromatographic columns are a valuable option for natural products that are difficult to separate by conventional means.
