A two-dimensional g-C3N4 nanosheet for high loading and sustained release of water-soluble drug salvianolic acid B
10.16438/j.0513-4870.2019-0878
- VernacularTitle:一种二维C3N4纳米片用于水溶性药物丹酚酸B的高负载及缓释作用
- Author:
Yong-shi LIANG
1
;
Chen-chen DING
1
;
Pei LUO
2
;
Zhi-feng ZHANG
2
;
Lu WEN
3
;
Gang CHEN
1
Author Information
1. School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China; Guangdong Provincial Key Laboratory of Advanced Drug Delivery, Guangdong Pharmaceutical University, Guangzhou 510006, China; Guangdong Provincial Engineering Center of Topical Precise Drug Delivery System, Guangdong Pharmaceutical University, Guangzhou 510006, China
2. State Key Laboratory for Quality Research in Chinese Medicines, Macau University of Science and Technology, Macau 000853, China
3. School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China
- Publication Type:Research Article
- Keywords:
graphitic carbon nitride;
salvianolic acid B;
two-dimensional layer material;
ater-soluble drug;
sustained release
- From:
Acta Pharmaceutica Sinica
2020;55(6):1296-1305
- CountryChina
- Language:Chinese
-
Abstract:
Delivering water-soluble drugs via carriers often causes problems such as low loading and rapid releasing, so it is an urgent need to construct a high-load sustained-release drug delivery system for the clinical application of water-soluble drugs. Two-dimensional layered nanomaterials exhibit great potential in drug delivery due to their high specific surface area. In this study, bulk graphitic carbon nitride (b-g-C3N4) was obtained by calcination of urea. Graphitic carbon nitride nanosheets (g-C3N4-NS) were made from an alkali chemical-ultrasonic-assisted stripping process. Scanning electron microscopy, transmission electron microscopy and atomic force microscopy were adopted to observe the morphological characteristics of g-C3N4-NS, while the structural characteristics of g-C3N4-NS were analyzed by X-ray diffractometer and Fourier transform infrared spectroscopy. Ultraviolet spectrometry and fluorescence spectrometry were used to investigate the optical properties of g-C3N4-NS, and scanning electron microscopy and X-ray diffractometer were employed to investigate the stability of g-C3N4-NS. Polyethyleneimine (PEI) was applied in the study to functionally modify g-C3N4-NS, and salvianolic acid B (Sal B) was used as a water-soluble drug model to investigate the loading capacity and drug releasing behavior of g-C3N4-NS. The results showed that g-C3N4-NS had a sheet structure, and it is easy to self-assemble in layers in the ionic environment to create flocculating settling. PEI modification can lead to the switching in the surface charge of g-C3N4-NS and significantly improve its stability. The results of cytotoxicity test and zebrafish embryo toxicity test showed that the toxicity was low when the concentration of PEI-g-C3N4-NS was less than 800 μg·mL-1. The large specific surface area and surface charge of PEI-g-C3N4-NS allow the maximum load factor over Sal B to reach 327.4%. In addition, PEI-g-C3N4-NS can continuously release drugs slowly, with a cumulative release rate of 79.2% in seven straight days. The release process conforms to the Higuchi equation. In summary, g-C3N4-NS modified by PEI exhibits good biocompatibility and high stability, and shows great potential in high-load and sustained-release applications of water-soluble drugs.
