Fluorinated hyperbranched poly(amido amine)s as carriers of influenza DNA vaccine
10.16438/j.0513-4870.2019-0713
- VernacularTitle:氟化超支化聚酰胺-胺作为流感DNA疫苗递送载体的研究
- Author:
Xue-lian FAN
1
;
Yue-hua XU
1
;
Gang CHEN
1
Author Information
1. Institute of Comparative Medicine, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,Yangzhou University, Yangzhou 225009, China
- Publication Type:Research Article
- Keywords:
fluorination;
polycation;
influenza;
DNA vaccine;
polyplexes
- From:
Acta Pharmaceutica Sinica
2020;55(6):1282-1287
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to synthesize fluorinated hyperbranched poly(amido amine)s for the delivery of influenza DNA vaccine. Hyperbranched poly(amido amine)s (HP) were synthesized by using Michael-type polyaddition and then fluorinated to obtain fluorinated polymers (F-HP). The target gene was amplified by designing specific primers to construct the eukaryotic expression plasmid of influenza viral PR8 nucleoprotein (NP) gene as DNA vaccines. Then the polyplexes (F-HP/NP) were prepared by the electrostatic interactions between polymers and plasmid. The results suggested that the molecular weight of HP was 59.7 kDa and polydispersity index (PDI) was 2.67. The fluorine content in F-HP was found to be 20% (w/w). Transmission electron microscopy (TEM) revealed that the polyplexes had spherical shapes with sizes around 100 nm (w/w 5-10) and decreased with increasing w/w ratios. F-HP polyplexes showed improved cell uptake, lysosomal escape and elevated expression levels of NP in vitro than polyplexes based on HP. Finally, the in-vivo immunization by F-HP/NP polyplexes triggered improved CD8+ T cell responses. This study suggests that fluorinated hyperbranched poly (amido amine)s represent one of the effective carriers for DNA vaccine delivery. The animal experiments were approved by the Experimental Animal Ethics Committee of Yangzhou University.
