Expression and clinical significance of serum miRNAs in patients with rheumatoid arthritis
10.13602/j.cnki.jcls.2019.12.09
- VernacularTitle:血清miRNAs在类风湿关节炎患者中的表达及临床意义
- Author:
Rui LIU
1
;
Dong SHEN
2
;
Qiuhong LIU
1
;
Dawei CUI
3
Author Information
1. Department of Rheumatism, Suzhou TCM Hospital Affiliated to Nanjing University of Chinese Medicine
2. Department of Laboratory Medicine, Affiliated Suzhou Science and Technology Town Hospital of Nanjing Medical University
3. Department of Blood Transfusion, the First Affiliated Hospital, College of Medicine, Zhejiang University
- Publication Type:Journal Article
- Keywords:
rheumatoid arthritis;
serum miRNAs;
DAS28;
rheumatoid factor;
erythrocyte sedimentation rate
- From:
Chinese Journal of Clinical Laboratory Science
2019;37(12):915-918
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression levels and clinical significance of serum miRNAs, including miR-146a-5p, miR-155-5p and miR-124a-3p, in patients with rheumatoid arthritis (RA).
Methods:The clinical data, whole blood and serum samples from 39 RA patients hospitalized in our hospital during October 2016 and October 2018 were collected. Whole blood specimens were used to determine erythrocyte sedimentation rate (ESR), and serum samples were used to detect rheumatoid factor (RF), C-reaction protein (CRP) and miRNAs. The expression levels of serum miR-146a-5p, miR-155-5p and miR-124a-3p in RA patients were detected by SYBR Green real-time fluorescence quantitative PCR.
Results:The expression levels of serum miR-146a-5p (1.742±1.058) in RA patients before treatment were significantly higher than that in healthy controls (HC, 1.045±0.772), which were positively correlated with DAS28 scores (r=0.836 5,P=0.004 5), ESR (r=0.437 2, P=0.032 5) and RF levels (r=0.733 6,P=0.013 7). However, the expression levels of serum miR-155-5p (U=42.00,P=0.032 9) and miR-124a-3p (U=44.5,P=0.044 5) in RA patients were significantly lower than that in HC, and the expression levels of serum miR-155-5p were negatively correlated with RF levels (r=-0.445 3,P=0.031 6), and the expression levels of serum miR-124a-3p were negatively correlated with DAS28 scores (r=-0.538 7,P=0.025 8) and RF levels (r=-0.436 5,P=0.046 3). After treatment, the expression levels of serum miR-146a-5p (U= 60.00,P=0.003 8) in RA patients were significantly decreased, while the expression levels of serum miR-155-5p (U=64.00,P=0.005 9) and miR-124a-3p (U=85.00,P=0.042 2) were significantly increased.
Conclusion:The abnormal expression levels of serum miR-146a-5p, miR-155-5p and miR-124a-3p in RA patients have potential clinical significance for the diagnosis of RA.
