Inhibitory effect of heat shock pretreated rat bone marrow mesenchymal stem cells on chemotherapy-induced ovarian granulocyte apoptosis
10.16571/j.cnki.1008-8199.2020.04.005
- VernacularTitle: 热休克预处理大鼠骨髓间充质干细胞对化疗诱导卵巢颗粒细胞凋亡的抑制作用
- Author:
Qing-ru WANG
1
;
Xin-ran LI
1
;
Qing WANG
2
;
Jia-xin XIE
1
;
Xia-fei FU
1
Author Information
1. Department of Obstetrics and Gynecology, Zhujiang Hospital of Southern Medical University, Guangzhou 510220,Guangdong, China
2. Department of Obstetrics and Gynecology, Shenzhen Hospital of the University of Hong Kong,Shenzhen 518034,Guangdong,China
- Publication Type:Journal Article
- Keywords:
heat shock;
bone marrow mesenchymal stem cells;
granulosa cells;
apoptosis
- From:
Journal of Medical Postgraduates
2020;33(4):363-369
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveBone marrow-derived mesenchymal stem cells (MSCs) can promote ovarian angiogenesis, improve ovarian insufficiency caused by chemotherapy, and repair ovarian function, while heat shock pretreatment can reduce the apoptosis rate of stem cells and improve the therapeutic effect of stem cells. This study aims to investigate the effect of heat shock pretreatment on MSCs, and further study the effect of heat shock pretreated mesenchymal stem cells on chemotherapy-induced apoptosis of ovarian granulosa cells.Methods1. The bone marrow-derived MSCs of rats were isolated, cultured and identified, and pretreated within a 42 °C water bath for one hour. 2. Cisplatin (5 mg/L) was added to MSCs to simulate the local microenvironment of chemotherapy. MSCs were divided into four groups: blank control group, heat shock control group, model group, and heat shock model group. The effects of heat shock pretreatment on the proliferation, apoptosis and survival rate of MSCs were investigated by CCK-8 method, Hoechst33342/PI, and flow cytometry. 3. We isolate and culture rat ovarian granulosa cells (GCs) to establish an in vitro model of GCs injury under the induction of cisplatin (5 mg/L). The experiment was carried out in four groups: a control group, model group, MSCs model group, HS-MSCs model group. The apoptosis and survival rate were detected by Hoechst33342/PI and flow cytometry, respectively.Results1. The proliferation level and survival rate of MSCs in the heat shock control group were significantly higher than those in the other three groups, and the apoptosis rate was significantly lower than the other three groups (P<0.05). Compared to the model group, the proliferation level of the heat shock model group was significantly increased, and the apoptosis rate was significantly decreased (P<0.05), and the cell survival rate increased; 2. The apoptosis rate of GCs in the HS-MSCs model group was significantly lower than that in the other three groups. Compared to the MSCs model group, the apoptosis rate of GCs in the HS-MSCs model group was significantly decreased (P<0.05).ConclusionHeat shock pretreatment can increase the proliferation level and survival rate of MSCs, and reduce its apoptosis rate. Heat shock pretreated stem cells can effectively inhibit chemotherapy-induced apoptosis of ovarian granulosa cells.
