Optimization of Ultrasonic-assisted Ethanol- （NH4）2SO4 Aqueous Two-phase Extraction of Citrusinol from Desmo- dium caudatum by Box-Behnken Design-response Surface Methodology
- VernacularTitle:Box-Behnken设计-响应面法优化小槐花中柠檬酚的超声辅助乙醇-硫酸铵双水相提取工艺
- Author:
Zhoufeng HUANG
1
;
Xiaoxi HU
2
;
Jianyou HUANG
2
;
Xiao TAN
2
;
Guoshou LU
2
;
Xinqiao LIANG
3
Author Information
1. Guangxi Zhuang Autonomous Region Institute of Traditional Medical and Pharmaceutical Sciences，Nanning 530022，China;Guangxi Key Laboratory of Traditional Chinese Medicine Quality Standards，Nanning 530022，China
2. Guangxi Zhuang Autonomous Region Institute of Traditional Medical and Pharmaceutical Sciences，Nanning 530022，China
3. College of Pharmacy，Guangxi University o f TCM，Nanning 530022，China
- Publication Type:Journal Article
- Keywords:
Desmodium caudatum;
Citrusinol;
Aqueous two-phase extraction;
Ultrasonic extraction;
Box-Behnken design
- From:
China Pharmacy
2020;31(9):1085-1090
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To optimize ultrasonic-assisted ethanol-（NH4）2SO4 aqueous two-phase extraction technology of citru- sinol from Desmodium caudatum . METHODS ：Using the content of citrusinol as indexes ，with ethanol volume fraction ， solid-liquid ratio ，（NH4）2SO4 addition amount ，ultrasonic time and ultrasonic temperature as factors ，based on the single factor tests，Box-Behnken design-response surface methodology was used to optimize the extraction technology of citrusinol. RESULTS ： The optimized extraction technology of citrusinol included that ethanol volume fraction was 95.35％，the solid-liquid ratio was 1∶50.35 （g/mL），（NH4）2SO4 addition amount was 4.49 g，ultrasonic time was 48.7 min，ultrasonic temperature was 57.6 ℃. In 3 times of validation tests ，the extraction rates of citrusinol were 0.637 8，0.638 4，0.625 4 mg/g，respectively，which was close to predicted value（0.630 5 mg/g）. CONCLUSIONS ：The optimized ultrasonic-assisted ethanol- （NH4）2SO4 aqueous two-phase extraction technology is stable and feasible ，and can be used for the extraction of citrusinol from D. caudatum .
