Determination of britanin content in different parts of Inula linariifolia Turcz by HPLC
10.12206/j.issn.1006-0111.202001049
- VernacularTitle:HPLC法测定条叶旋覆花不同部位中线叶旋覆花内酯A的含量
- Author:
Yubin LI
1
;
Ji YE
1
;
Weidong ZHANG
1
Author Information
1. Department of Natural Medicinal Chemistry, School of Pharmacy, Naval Medical University, Shanghai 200433, China.
- Keywords:
Inula linariifolia Turcz.;
dried aerial parts;
capitulum;
britanin;
content determination
- From:
Journal of Pharmaceutical Practice
2020;38(3):245-249
- CountryChina
- Language:Chinese
-
Abstract:
Obejctive To determine and compare britanin content in dried aerial parts and capitulum of Inula linariifolia Turcz from 7 different origins by HPLC. Methods Analysis was performed on Agilent Zorbax SB C18 (250 mm×4.6 mm, 5 μm). Acetonitrile and water were used as mobile phase for gradient elution at 1.2 ml/min. Column temperature was 30 ℃ and the detection wavelength at 212 nm. Results The results meet the requirements of the method validation in 2015 edition of Chinese Pharmacopeia. The average britanin content in dried aerial parts of Inula linariifolia Turcz is 0.125% vs 0.732% in capitulum, which is significantly different. Conclusion The established method is simple and convenient. It can be used for quality control of Inula linariifolia Turcz.
