The effect of four compounds of astragalus isoflavones on the differentiation of PC 12 cells
10.12206/j.issn.1006-0111.202001045
- VernacularTitle:4个黄芪异黄酮类化合物对PC 12细胞分化的影响
- Author:
Qiuling WU
1
;
Yanqiong CHENG
2
;
Aijun LIU
2
;
Weidong ZHANG
3
Author Information
1. School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China.
2. Department of Pharmacology, School of Pharmacy, Naval Medical University, Shanghai 200433, China.
3. School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China;Department of Natural Medicinal Chemistry, School of Pharmacy, Naval Medical University, Shanghai 200433, China.
- Keywords:
calycosin;
formononetin;
calycosin-7-glucoside;
ononin;
PC 12 cells;
cell differentiation
- From:
Journal of Pharmaceutical Practice
2020;38(3):232-236
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of calycosin, formononetin, calycosin-7-glucoside and ononin on PC 12 cells differentiation. Methods PC 12 cells were cultured and treated with different concentrations of nerve growth factor (NGF), calycosin, formononetin, calycosin-7-glucoside and ononin for 5 days, once a day, 3 times in a row. The neurite outgrowth of PC 12 cells was observed and the expression of β III-tubulin were measured by immunofluorescence. Results Compared with the vehicle group, neurite outgrowth and the expression of β III tubulin in PC 12 cells had not promoted by calycosin, formononetin, calycosin-7-glucoside and ononin (0.01-10.00 μmol/L). Conclusion PC 12 cells differentiation could not be induced by calycosin, formononetin, calycosin-7-glucoside and ononin.
