Asiatic acid enhances the chemosensitivity of U87MG glioma cells to paclitaxel through inhibiting the expression of drug resistance related proteins
10.3872/j.issn.1007-385x.2018.04.004
- VernacularTitle:积雪草酸通过抑制耐药相关蛋白表达增强U87MG胶质瘤细胞对紫杉醇 的敏感性
- Author:
ZHANG Lei
1
;
CHEN Lei
2
;
CHEN Jie
1
;
YANG Jingjing
1
Author Information
1. a. Department of Pediatrics, People’s Hospital of Shizhong District of Ji’nan City, Ji’nan 250023, Shandong, China
2. b. Department of Oncology, People’s Hospital of Shizhong District of Ji’nan City, Ji’nan 250023, Shandong, China
- Publication Type:Journal Article
- Keywords:
asiatic acid;
glioma;
drug resistance;
chemosensitivity;
paclitaxel;
U87MG
- From:
Chinese Journal of Cancer Biotherapy
2018;25(4):340-345
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective: To explore the inhibitive effect of asiatic acid (AA) on paclitaxel (PTX)-resistant glioma cells and its possible mechanism. Methods: The effects of AA on the proliferation and apoptosis of glioblastoma U87MG cells were detected by CCK-8 assay, Real-time quantitative polymerase chain reaction (qPCR) and Western blotting. The drug-resistant glioma cell line PR-U87MG was established by culturing the cells in concentration-increasing PTX. With U87MG cells as control, the PTX-resistance of PR-U87MG cells was confirmed using CCK-8 assay, and the mRNA and protein levels of MDR1 and LRP were measured with qPCR and western blotting. PR-U87MG cells were treated with AA, PTX or AA+PTX, and then the cell viability and apoptosis of each group were measured with CCK-8 assay, qPCR and Western blotting. Results: PTX-resistant PR-U87MG cell line was successfully established. AA inhibited the viability of U87MG and PR-U87MG cells in a dose-dependent manner (P<0.01) and significantly promoted their apoptosis (P<0.01). Compared with the group treated with AA or PTX alone, the group treated with the combination of AA and PTX had significantly decreased protein levels of PARP1 (P<0.01), drug-resistant related proteins (Pgp-1 and LRP [lung resistance protein], all P< 0.01), and markedly increased caspase 3 (P<0.01). Conclusion: AA could effectively enhance the sensitivity of U87MG cells to PTX, and the mechanism may be related to the suppressed expression of drug efflux-associated proteins Pgp-1 and LRP.
- Full text:20180404.pdf
