Mechanism research of srtA gene on the oxidation tolerance of Streptococcus mutans
10.12016/j.issn.2096-1456.2020.05.004
- Author:
HE Yuanli
1
;
REN Biao
1
;
CHEN Xuan
1
;
ZOU Ling
2
Author Information
1. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & West China Hospital of Stomatology, Sichuan University
2. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Conservative Dentistry and Endodontics, West China Hospital of Stomatology, Sichuan University
- Publication Type:Journal Article
- Keywords:
Streptococcus mutans;
dental caries;
biofilm;
Sortase A;
srtA gene;
oxidative stress;
oxidation tolerance;
reactive oxygen species;
RNA-Seq;
two-component signal transduction systems
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2020;28(5):292-297
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of srtA on the oxidation tolerance of the Streptococcus mutans UA159 strain and to explore the potential mechanism.
Methods:The oxidation tolerance in the planktonic state and biofilm state were compared among UA159, the srtA-deleted strain and the complementary strain through oxidative tolerance experiments. The RNA-sequencing data from both the exponential and stationary phases of UA159 and the srtA-deleted strain were obtained by using the Illumina HiSeq 4 000 sequencing platform to determine the impact of srtA knockout on S. mutans genomic transcription. We compared the differences in the transcriptional expression of oxidative tolerance-related genes between the UA159 strain and the srtA gene deletion strain and further explored the intrinsic relationship between the changes in oxidative tolerance and the genetic transcriptome. qPCR was used to verify the changes in the expression level of oxidation tolerance-related genes.
Results :The oxidation tolerance of the srtA-deleted strain decreased significantly in both the planktonic state and the biofilm state compared to that of UA159 (P < 0.05). A total of 33 oxidation tolerance-related genes were differentially expressed according to transcriptome sequencing. There was no significant change in the expression of peroxide synthesis- and metabolic-related enzyme genes, but in the stationary phase samples, the two-component signal transcription systems lrgA, lrgB, and lytT were significantly downregulated (2.2- to 2.4-fold) in the srtA-deleted strain. qPCR further confirmed that in both the exponential and stationary phases, lrgB and lytT expression in the planktonic state was reduced 11.01-53.51-fold, while the expression of the other two-component system-encoding gene vicK was reduced by 6.57-10.88-fold (P < 0.001).
Conclusion:SrtA gene deletion did not change the expression level of peroxide synthesis-related and metabolic enzyme-encoding genes but downregulated the expression of the associated transcription regulation factors to reduce the oxidation tolerance of S. mutans.
- Full text:srtA基因调节变异链球菌氧化耐受的机制研究.pdf
