Effects of low temperature on trehalose and trehalase contents in Culex pipiens pallens
10.16250/j.32.1374.2018131
- VernacularTitle:低温对淡色库蚊海藻糖和海藻糖酶的影响
- Author:
Ben-Guang ZHANG
1
;
Ya-Ping LI
2
;
Rong MA
3
;
Peng CHENG
1
;
Xiu-Xia GUO
1
;
Hai-Fang WANG
1
;
Li-Juan LIU
1
Author Information
1. Shandong Institute of Parasitic Diseases, Shandong First Medical University & Shandong Academy of Medical Sciences, Jining 272033, China
2. Jinxiang County Bureau of Agriculture, Shandong Province, China
3. Shandong Institute of Parasitic Diseases, Shandong First Medical University & Shandong Academy of Medical Sciences, Jining 272033, China; Jining No. 1 People’s Hospital, Shandong Province, China
- Publication Type:Journal Article
- Keywords:
Culex pipiens pallens;
Trehalose;
Trehalase;
Low temperature
- From:
Chinese Journal of Schistosomiasis Control
2019;31(5):510-512
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the effect of low temperature on trehalose and trehalase levels in Culex pipiens pallens. Methods The fourth instar larvae and female adult mosquitoes of Cx. pipiens pallens were exposed at 4 ℃ for 0, 1, 3, 6, 12, 18, 24 h and 0, 1, 3, 6, 12, 18, 24, 36, 48, 72 h, respectively. Then, the trehalose and trehalase contents were detected by enzyme-linked immunosorbent assay (ELISA) in mosquitoes. Results The contents of trehalose and trehalase significantly increased in the larval and female adult mosquitoes post-exposure to low temperature. The changing trend of trehalose levels was consistent in the larval and female adult mosquitoes, and the highest levels were (2.458 8 ± 0.379 2) mg/g and (2.825 7 ± 0.211 1) mg/g 3 h post-exposure to low temperature, respectively. The trehalose and trehalase levels fluctuated greatly within the first 6 h post-exposure to low temperature. Following adaptation for a period of time, the trehalose and trehalase levels remained at a relatively high level. Conclusion Low temperature may induce the production of trehalose and trehalase in Cx. pipiens pallens, and the trehalose and trehalase may play an important role in the improvement of the cold resistance.
