Effect of transfusing blood components containing Babesia microti on B. microti infection in BALB/c mice
10.16250/j.32.1374.2019111
- VernacularTitle:输入染虫成分血对BALB/c小鼠感染田鼠巴贝虫的影响
- Author:
Hui-Min SHEN
1
;
Yu-Chun CAI
2
;
Jia-Xu CHEN
2
;
Kui-Yang ZHENG
3
Author Information
1. Department of Pathogenic Biology and Immunology, Xuzhou Medical University, Jiangsu Provincial Key Laboratory of Immunity and Metabolism, Xuzhou 221004, China; National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, China
2. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, China
3. Department of Pathogenic Biology and Immunology, Xuzhou Medical University, Jiangsu Provincial Key Laboratory of Immunity and Metabolism, Xuzhou 221004, China
- Publication Type:Journal Article
- Keywords:
Babesia microti;
Blood component;
Blood transfusion;
Mouse
- From:
Chinese Journal of Schistosomiasis Control
2019;31(4):423-426
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of intravenous injection of different blood components containing Babesia microti on B. microti infection in mice. Methods Healthy mice were infected with B. microti, and then blood samples were collected from the mouse orbit to prepare whole blood, serum-free blood components and pure red blood cells containing B. microti. Twenty seven BALB/c mice were divided into three groups, including the whole blood group, the serum-free blood component group and the pure red blood cell group, of 9 mice in each group, and then, each group was divided into three subgroups, of 3 mice in each subgroup, which were injected with 100 μL of blood components containing B. microti at concentrations of 9.00, 0.90, 0.09 B. microti parasites/μL (900, 90, 9 B. microti parasites) via the tail vein, respectively. Blood samples were collected from the mouse tail tip every other day since one day post-injection to prepare thin blood smears. Following Giemsa staining of blood smears, B. microti infection was identified in red blood cells using microscopy. Results Following injection of 900 B. microti parasites, B. microti was identified in the peripheral blood in the whole blood group and the serum-free blood component group 3 days post-injection, and the density of B. microti parasites started to increase 15 days post-injection and peaked 21 days post-injection, with 2.21% and 1.76% rates of B. microti infection in red blood cells, respectively. Subsequently, the density of B. microti parasites declined, and the percentage of B. microti infection in red blood cells tended to be 0 31 days post-injection. During the study period, no B. microti was found in the peripheral blood in the pure red blood cell group. Following injection of 90 B. microti parasites, B. microti was identified in the peripheral blood in the whole blood group 3 days post-injection, and the density of B. microti parasites increased 15 days post-injection and peaked 21 days post-injection, with a 1.35% rate of B. microti infection in red blood cells, while the percentage of B. microti infection in red blood cells tended to be 0 31 days post-injection. During the study period, no B. microti was detected in the peripheral blood in the serum-free blood component group or the pure red blood cell group. Following injection of 9 B. microti parasites, no B. microti was detected in the peripheral blood in the whole blood group, the serum-free blood component group or the pure red blood cell group. Conclusion Blood components and dose of B. microti parasites may affect intravenous injection of B. microti injection in mice, and transfusion of blood components may case a risk of Babesia infection.
