Study on the mechanisms underlying overwintering of Culex pipiens pallens using iTRAQ-based quantitative proteomic analysis
10.16250/j.32.1374.2018301
- VernacularTitle:基于iTRAQ的定量蛋白质组学技术探索淡色库蚊越冬机制
- Author:
Qian ZHANG
1
;
Gui-Hong SHI
2
;
Xiu-Xia GUO
2
;
Hai-Fang WANG
2
;
Jie ZHANG
2
;
Chong-Xing ZHANG
2
Author Information
1. Shandong Institute of Parasitic Diseases, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250022, China; School of Medicine and Life Sciences, University of Jinan Shandong Academy of Medical Sciences, China; Jining Municipal Hospital of Traditional Chinese Medicine, Shandong Province, China
2. Shandong Institute of Parasitic Diseases, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250022, China
- Publication Type:Journal Article
- Keywords:
Culex pipiens pallens;
Adult mosquito overwintering;
Quantitative proteomics;
Isobaric tags for relative and absolute quantification (iTRAQ)
- From:
Chinese Journal of Schistosomiasis Control
2019;31(2):160-164
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the difference of protein expression between the post-overwintering stage and the diapauses preparation stage in Culex pipiens pallens, so as to reveal the mechanisms underlying the overwintering diapause of Cx. pipienspallens. Methods A quantitative proteomic analysis was performed in Cx. pipiens pallens before and after overwintering diapause by using isobaric tags for relative and absolute quantification (iTRAQ) labeling. Results A total of 244 differentially expressed proteins were identified in Cx. pipiens pallens before and after overwintering diapause, including 126 up-regulated proteins and 118 down-regulated proteins. iTRAQ-based quantitative proteomic analysis revealed that these differentially expressed proteins were linked to function and energy production and conversion, lipid metabolism, remodeling of cytoskeleton, carbohydrate metabolism, protein transport, molecular chaperones, stress tolerance and metabolic enzymes. Conclusions This is the first study to identify the overwintering diapause-related proteins in Cx. pipiens pallens using proteomics tools, which reveals KEGG pathways and GO terms associated with the overwintering diapauses of Cx. pipiens pallens. Our findings provide additional understandings pertaining to the mechanisms underlying the overwintering diapauses of Cx. pipiens pallens.
