Protective effect of bisoprolol against hypoxia/reoxygenation injury of cardiac microvascular endothelial cells
10.16571/j.cnki.1008-8199.2020.01.007
- VernacularTitle: 比索洛尔对小鼠心肌微血管内皮细胞缺氧复氧损伤的保护作用
- Author:
Shu-hong CAI
1
;
Zhi-wen FAN
2
;
Xiao-ping QIN
2
;
Yun-wen CHEN
2
;
Jin-hu ZHANG
2
;
Qiu-ming ZHOU
2
;
Chao ZENG
2
Author Information
1. Divison of Medical Affairs, No
2. Department of Cardiology, The 74th Group Army Hospital of People’s Liberation Army, Guangzhou 510318, Guangdong, China
- Publication Type:Journal Article
- Keywords:
cardiac microvascular endothelial cells;
bisoprolol;
hypoxia/reoxygenation injury;
oxidative stress
- From:
Journal of Medical Postgraduates
2020;33(1):38-43
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the protective effect of bisoprolol against hypoxia/reoxygenation injury of cardiac microvascular endothelial cells and explore the mechanism. Methods Left ventricular of cardiac microvascular endothelial cells (CMECs) were isolated from 8-week-old male C57BL/6N mice. CMECs were randomized into four groups: control group, vehicle group, hypoxia/reoxygenation group (H/R group), hypoxia/reoxygenation + bisoprolol group. The level of cell proliferation, apoptosis, superoxide anion, Cleaved caspase-3 and Nox2 expression were measured in each group. Results Compared with control group, H/R group had lower cell proliferation, higher apoptotic level, more superoxide anion level and the expression of Cleaved caspase-3 and Nox2 (P < 0.05). Furthermore, bisoprolol reversed hypoxia/reoxygenation-induced the decreased cell proliferation, the increased apoptosis, superoxide anion level, Cleaved caspase-3 and Nox2 expression (P < 0.05). Conclusion Bisoprolol can protect CMECs against hypoxia/reoxygenation injury by reducing the expression of Nox 2 that decreases oxidative stress.
