Comparative analysis of serum IgE detected by ImmunoCAP and EUROLine assays
10.16571/j.cnki.1008-8199.2018.12.010
- VernacularTitle: 采用ImmunoCAP法和EUROLine法检测血清IgE的对比分析
- Author:
Zhi-feng HUANG
1
;
Hai-sheng HU
1
;
Xiang-wei ZOU
1
;
Wen-ting LUO
1
;
Ze-hong WU
1
;
Yue-xiao TAO
1
;
Bao-qing SUN
1
Author Information
1. State Key Laboratory of Respiratory Diseases, Guangzhou Institute of Respiratory Health, Department of Allergy and Clinical Immunology, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, Guangdong,China
- Publication Type:Journal Article
- Keywords:
allergen;
specific IgE;
EUROLine;
ImmunoCAP
- From:
Journal of Medical Postgraduates
2018;31(12):1276-1280
- CountryChina
- Language:Chinese
-
Abstract:
Objective The diagnosis of allergic diseases mainly relies on the allergen skin test or in vitro specific IgE test. This study was to explore the diagnostic efficiency of EUROLine and its application in southern China by reference to the standard method of the immunoenzymatic capsulated hydrophilic carrier polymer (ImmunoCAP).Methods Using the EUROLine system, we examined 12 common specific IgEs (sIgE) from 283 patients with multiple sensitizations in the First Affiliated Hospital of Guangzhou Medical University, including cockroach (i6), dog dander (e2), cat dander (e1), Mugwort (w6), ragweed (w1), dust mites (ds1), chicken protein (f1), milk (f2), crab (f23), shrimp (f24), peanut (f13) and soybean (f14).Results EUROLine showed that house dust mite allergens were the main aeroallergens in southern China, with a positive rate of 66.3%, while egg white had the highest positive rate (53.2%) in food allergens. The overall rate of the allergens detected by EUROLine was consistent to that of ImmunoCAP by 60.8%-90.1%, the positive rate by 63.3%-93.6%, and the negative rate by 54.5%-95.2%. The Kappa value of EUROLine was 0.203-0.702 in detecting each allergen and >0.6 with cat hair, egg white, peanut, milk and mugwort. The rank correlation coefficient between ImmunoCAP and EUROLine was >0.7 for dust mite combination, cat hair, mugwort, egg white, milk, peanut and crab allergens, with the highest consistency rate for egg white (93.17% \[191/205\]), the lowest for shrimp (70.83% \[170/240\]), and an overall consistency rate of 82.68% (1542/1865).Conclusion The EUROLine system has a high diagnostic performance, and it is inexpensive, efficient and applicable in the detection of allergens in southern China.
