Screening and Verification of Long Non-coding MAPT-AS1 and Their Co-expressed Genes in Breast Cancer Survival
- VernacularTitle:乳腺癌生存期相关长非编码 MAPT-AS1 及其共表达基因筛选与验证
- Author:
Jia-rui XU
1
;
Dong-dong LIU
1
;
Bei-bei LI
1
;
Meng-lin CHEN
2
;
Wan WANG
1
;
Kai-ling HUANG
2
;
Zhou-xia ZHENG
2
;
Jian-hua XU
3
Author Information
1. Department of Laboratory Science，the Second Affiliated Hospital of Guangzhou University of Chinese Medicine， Guangzhou 510120，China
2. Guangzhou Mendel Genomics and Medical Technology Co. Ltd，Guangzhou 510535， China
3. Shunde Hospital of Guangzhou University of Chinese Medicine，Foshan 528000，China
- Publication Type:Journal Article
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2019;40(3):467-472
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】To screen survival-related differential expression of long non-coding RNA（lncRNA）and its co-expressed genes in breast cancer patients and to verify their expression in breast cancer cells.【Methods】RNA-seq data of 943 cases（837 breast cancer + 106 normal controls）by the TCGA database were screened，and found that long non-coding MAPT-AS1 highly expressed，and breast cancer patients had longer survival. The long non-coding MAPT- AS1 overexpression and interference plasmid was constructed，and the constructed plasmid was transfected into breast cancer cell line T47D，and the stably expressed T47D cell line was screened by puromycin. The expression of long non-coding MAPT-AS1 and its co-expressed genes was verified by the methods of RT-qPCR.【Results】Fluorescence microscopy and RT-qPCR confirmed that the long non-coding MAPT-AS1 overexpression and interference-transfected breast cancer cell line T47D were successfully constructed，and the long non-coding MATS-AS1 interference fragment shRNA3 with the highest interference efficiency was screened. The expression of MAPT ，MAPT- IT1 and NXNL2 in the co-expressed gene was decreased after transfection of the shRNA3 interference fragment ，which was consistent with the expression trend of the long non-coding MAPT-AS1.【Conclusion】The long non-coding MAPT-AS1 overexpression and interference plasmid transfected breast cancer cell line T47D were successfully constructed，and the expression of the co- expressed gene was consistent with the database. The study laid the foundation for further study of the mechanism of action of long non-coding MAPT-AS1 gene in breast cancer.
