Long Non-Coding RNA Expression Profile in Vascular Endothelial Cells before and after Infected by Dengue Virus Type Ι
- VernacularTitle:I型登革热病毒感染前后血管内皮细胞长链非编码RNA表达谱分析
- Author:
Bao-jia ZHENG
1
;
Zhen-you JIANG
1
;
Lu-lu SI
2
;
Qian-fang GUO
2
;
Li-fang JIANG
2
;
Jun-mei ZHOU
2
;
Hui WANG
1
Author Information
1. Department of Microbiology and Immunology，School of Basic Medical Sciences，Jinan University，Guangzhou510632，China
2. Key Laboratory of Tropical Diseases Control，Ministry of Education，and Deparment of Medical Microbiology，Zhongshan Medical College，Sun Yat-sen University，Guangzhou 510080，China
- Publication Type:Journal Article
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2019;40(2):228-236
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To use high- throughout sequencing technologies for examining differentially expressed long non-coding RNA（lncRNA）in human umbilical vein endothelial cell（HUVEC）infected by dengue virus type Ι（DENV-1）， to analyze and explore the potential molecular mechanisms of HUVEC dysfunction or damage.【Methods】After 24 hours of DENV-1 infection，RNA samples were extracted from control groups and viral groups. Sequencing and the differentially expressed lncRNAs were screened ，and then GO and KEGG enrichment analysis were conducted and a co- expression network map was constructed.【Results】In contrast to the control group，there were 2 623 lncRNA expressed differently， among which 1 441 were up-regulated，while 1 182 were down-regulated. It was found that the differentially expressed lncRNA and the predicted corresponding target genes were mainly distributed in the regions of biological processes of antigen presentation，interferon synthesis，apoptosis and cell adhesion. 【Conclusion】After HUVEC were infected with DENV-1，lncRNA expression profile changes significantly，which is closely related to the occurrence and development of dengue hemorrhagic fever/dengue shock syndrome（DHF/DSS）.
