Establishment of HPLC Fingerprint of Pholidota chinensis and Its Cluster Analysis
- VernacularTitle:石仙桃药材HPLC指纹图谱的建立及聚类分析
- Author:
Miao ZHANG
1
;
Long CHEN
1
;
Hua ZHU
1
;
Li LI
1
;
Fangfang DA
1
;
Li LONG
1
;
Ruiting HE
1
Author Information
1. Key Lab of Zhuang Yao Medicine，Guangxi University of TCM，Nanning 530200，China
- Publication Type:Journal Article
- Keywords:
Pholidota chinensis;
HPLC;
Fingerprint;
Cluster analysis
- From:
China Pharmacy
2019;30(13):1792-1795
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To establish HPLC fingerprint of Pholidota chinensis and cluster analysis the samples， and to provide reference for quality evaluation of P. chinensis. METHODS： HPLC fingerprints of 16 batches of P. chinensis from Guangxi， Guangdong and Fujian were established according to 2012 edition of Similarity Evaluation System for Chromatographic Fingerprint of TCM [chromatographic column was Agilent SB-aq column with mobile phase consisted of acetonitrile-0.05％ phosphoric acid solution （gradient elution）， at the flow rate of 1.0 mL/min. The detection wavelength of 220 nm， the column temperature was 30 ℃ and the sample volume was 5 μL].The cluster analysis was carried out by using SPSS 22.0 statistical software. RESULTS： Accoding to the established HPLC fingerprint， 11 common peaks were indentified， and the common peak 1 was identified as gastrodin； the similarity of 16 batch of samples was greater than 0.9. 16 batches of medicinal materials could be divided into 2 groups. CONCLUSIONS： The established HPLC fingerprint and cluster analysis can provide reference for the quality control of P. chinensis.
