Simultaneous Determination of Dehydrocorydine and Salvianolic Acid B in Shuangshen Tongguan Capsules by HPLC-dual Wavelength Switching Method
- VernacularTitle:HPLC-双波长切换法同时测定双参通冠胶囊中脱氢紫堇碱、丹酚酸B的含量
- Author:
Jin SUN
1
;
Peng ZHANG
1
;
Qing PENG
1
;
Shuo MENG
1
;
Junguo REN
1
;
Jianxun LIU
1
Author Information
1. Institute of Basic Medical Sciences，Xiyuan Hospital，China Academy of Chinese Medical Sciences，Beijing 100091，China
- Publication Type:Journal Article
- Keywords:
Shuangshen tongguan capsules;
HPLC-dual wavelength switching method;
Content determination;
Salvianolic acid B;
Dehydrocorydine
- From:
China Pharmacy
2019;30(7):886-889
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To develop an method for determining the contents of dehydrocorydine and salvianolic acid B in Shuangshen tongguan capsules simultaneously. METHODS： The HPLC-dual wavelength switching method was used. The determination was performed on Waters symmetry C18 column with mobile phase consisted of acetonitrile-0.05％ phosphoric acid solution （gradient elution） at the flow rate of 1.0 mL/min， the detection wavelength was 286 nm （salvianolic acid B） and 336 nm （dehydrocorydine）. The column temperature was maintained at 25 ℃， and sample size was 10 μL. RESULTS： Under this condition， dehydrocorydaline and salvianolic acid B could be separated in baseline. The linear range of them were 0.157-1.259 μg and 0.391-3.131 μg （r＝0.999 9）. RSDs of precision， reproducibility and stability tests （within 24 h） were all lower than 2.00％ （n＝6-10）. The average recovery rates were 101.61％ and 102.85％ （RSD＝3.59％ and 2.85％， n＝6）. CONCLUSIONS： Established HPLC-dual wavelength switching method can be used for simultaneous determination of dehydrocorydine and salvianolic acid B in Shuangshen tongguan capsules. The method is simple and rapid， and can be used for the quality control of Shuangshen tongguan capsule.
