Inhibition effect and mechanism of IL-8 down-regulation on migration of esophageal squamous cell carcinoma KYSE170 cells
10.3872/j.issn.1007-385x.2018.08.006
- VernacularTitle:下调IL-8表达抑制食管鳞状细胞癌KYSE170细胞的迁移能力及其相关 机制
- Author:
YANG Qin
1
;
ZHANG Xiangmei
1
;
HE Ming
2
;
CHEN Xin
2
;
ZHAO Jidong
2
;
SANG Meixiang
1
;
ZHAO Lianmei
1
;
SHAN Baoen
1
Author Information
1. a. Scientific Research Center, the Fourth Hospital of Hebei Medical University
2. Department of Fifth Thoracic Surgery, the Fourth Hospital of Hebei Medical University
- Publication Type:Journal Article
- Keywords:
esophageal squamous cell carcinoma;
KYSE170 cells;
interleukin-8;
migration
- From:
Chinese Journal of Cancer Biotherapy
2018;25(8):785-790
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of interleukin-8 (IL-8) on esophageal cancer cell line KYSE170, and to preliminarily explore its mechanism. Methods: siRNA targeting IL-8 was in vitro synthesized and transfected into KYSE170 cells by lipofectamine 2000. The efficiency of silencing was determined by Real-time PCR, Western blotting and ELISA. Morphological changes of KYSE170 cells were observed microscopically. Scratch assay was performed to observe the cell migration ability. CCK-8 assay was used to detect the cell proliferation ability. Western blotting was used to detect the expressions of IL-8 receptor and JAK2-STAT3 signaling pathway related proteins. Results: Compared with the negative control group, the mRNA and protein expressions of IL-8 in KYSE170 cells were all significantly decreased after IL-8 silencing (P<0.01), and IL-8 secretion was significantly reduced (P<0.01).After IL-8 gene silencing, the migration capacity of KYSE170 cells was significantly weakened (P<0.01), while no significant changes in cell proliferation was detected. The expression of IL-8 receptor 2 (CXCR2) and transfer-related protein WASF3 were significantly decreased (P< 0.05), while the expression of IL-8 receptor 1 (CXCR1) was not significantly changed; the expressions of p-JAK2 and p-STAT3 protein in JAK2-STAT3 signaling pathway were significantly decreased (all P<0.01). Conclusion: Knock-down of IL-8 inhibits the migration of esophageal cancer KYSE170 cells, and the mechanism may be related with the alteration of CXCR2 and its downstream JAK2STAT3 signaling pathway.
- Full text:20180806.pdf
