Screening and functional analysis of microRNA expression in HPV16-positive squamous carcinoma of the cervix
in the Uygur of southern Xinjiang.
10.11817/j.issn.1672-7347.2015.07.001
- Author:
Min YUAN
1
;
Jingxin CHENG
2
;
Yaxin LIU
1
;
Wei SU
1
;
Yu ZHANG
3
;
Yi ZHANG
3
Author Information
1. Department of Gynecology, Affiliated Tumor Hospital of Xinjiang Medical University, Ulumuqi 830011, China.
2. Department of Gynecology, Affiliated Tumor Hospital of Xinjiang Medical University, Ulumuqi 830011;Department of Obstetrics and Gynecology, Xiangya Hospital, Central South University, Changsha 410008, China.
3. Department of Obstetrics and Gynecology, Xiangya Hospital, Central South University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Carcinoma, Squamous Cell;
metabolism;
virology;
China;
Down-Regulation;
Female;
Human papillomavirus 16;
isolation & purification;
Humans;
MicroRNAs;
metabolism;
Papillomavirus Infections;
metabolism;
Real-Time Polymerase Chain Reaction;
Uterine Cervical Neoplasms;
metabolism;
virology
- From:
Journal of Central South University(Medical Sciences)
2015;40(7):701-709
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the differential expression of microRNA (miRNA) in HPV16-positive squamous carcinoma of the cervix in the Uygur of southern Xinjiang and to predict the target genes of the miRNAs.
METHODS:Samples of HPV16-positive squamous carcinoma of the cervix from 5 Uygurs were collected for miRNA microarray assay. The differentially expressed miRNAs were selected for further verification by real-time quantitative RT-PCR. The software, including targetscan, miRwalk, miRanda and pictar, were used to predict the target genes of the verified miRNAs.
RESULTS:Eighteen differentially expressed miRNAs were identified by miRNA microarray assay. The significantly differentially expressed miRNA-138 and miRNA-720 were verified by real-time quantitative RT-PCR. According to the prediction, the target genes for miRNA-138 were EZH2, LYPLA1, ARHGEF3, CLNS1A, EIF4EBP1, GNAI2, LIMK1, RHOC, ROCK2, SLC20A1, TERT, and H2AFX, while for miRNA-720 were EZH2, AGAP2, SPOCK2, FGF14, HNRNPA2B1, QKI, FOXG1, ACVR1B, DNMT3A, EPHB2, LATS2, KRAS, CCND2, NBN, ENAM, AMELX, PRNP, and CALB1.
CONCLUSION:miR-138 and miR-720 are the down-regulated target miRNAs in HPV16-positive squamous carcinoma of the cervix in the Uygur of southern Xinjiang. The common target gene for miR-138 and miR-720 is EZH2, which might be related to cervical squamous carcinoma invasion and metastasis.
