Effects of methylseleninic acid on cisplatin-resistant ovarian cancer cells (SKOV3/DDP) and the mechanisms.
10.11817/j.issn.1672-7347.2016.12.009
- Author:
Ying TAN
1
;
Qing FENG
1
;
Xin SUN
1
;
Min XUE
1
;
Ning JIANG
1
;
Xinliang DENG
1
Author Information
1. Department of Obstetrics and Gynecology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
- Publication Type:Journal Article
- MeSH:
Antineoplastic Agents;
pharmacology;
Carcinoma;
physiopathology;
Cell Line, Tumor;
physiology;
Cell Proliferation;
drug effects;
Cisplatin;
pharmacology;
Drug Resistance, Neoplasm;
drug effects;
genetics;
Female;
Humans;
Organoselenium Compounds;
pharmacology;
Ovarian Neoplasms;
physiopathology;
beta Catenin;
drug effects;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2016;41(12):1305-1311
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the reversal effect of methylseleninic acid on cisplatin (DDP)-resistant ovarian cancer cells and the underlying mechanisms.
Methods: SKOV3/DDP cells were incubated with cisplatin at different concentrations for 48 h, then the proliferation rate of SKOV3/DDP cells was detected by MTT assays, and the expression of β-catenin in SKOV3/DDP cells was examined by Western blot. The inhibitory effect of methyl-seleninic acid (MSA) combined with DDP at different concentrations on SKOV3/DDP cells was assayed by MTT method. Western blot was used to detect the expression of β-catenin protein in the cells.
Results: The inhibitory rate for proliferation in DDP-treated SKOV3/DDP cells with different concentrations is lower than that in the SKOV3 cells (P<0.05); β-catenin expression in SKOV3/DDP cells was significantly higher than that in the SKOV3 cells (P<0.05). The inhibitory rate for proliferation in SKOV3/DDP cells with different concentrations of MSA was increased with the increase in concentration (P<0.05). The inhibitory rate for proliferation in SKOV3/DDP cells with 2 or 6 μmol/L MSA plus cisplatin was lower than that in cisplatin alone group (P<0.05). β-catenin expression in SKOV3 /DDP cells with 2 or 6 μmol/L MSA plus cisplatin was higher than that in the cisplatin alone group (P<0.05).
Conclusion: MSA can reverse cisplatin resistance on SKOV3 / DDP cells, which may be related to the decrease in β-catenin expression.
