Performance effect of rabbit carotid artery treated with decellularization and photo-oxidation.
10.3969/j.issn.1672-7347.2013.05.010
- Author:
Feng KUANG
1
;
Zhonggui SHAN
;
Wenjun YOU
;
Xinmin ZHOU
;
Bangliang YIN
;
Xiaobiao ZHOU
Author Information
1. Department of Cardiac Surgery, First Affiliated Hospital of Xiamen University, Xiamen Fujian 361001,China.
- Publication Type:Journal Article
- MeSH:
Animals;
Blood Vessel Prosthesis;
Calcinosis;
prevention & control;
Carotid Arteries;
cytology;
transplantation;
Cell Separation;
methods;
Female;
Histocytological Preparation Techniques;
Male;
Oxidants, Photochemical;
pharmacology;
Oxidation-Reduction;
Rabbits;
Rats;
Rats, Sprague-Dawley;
Transplantation, Heterologous
- From:
Journal of Central South University(Medical Sciences)
2013;38(5):503-509
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To analyze and discuss the feasibility of rabbit carotid artery treated with decellularization and photo-oxidation.
METHODS:Sixty vascular slices of rabbit carotid artery were divided into a fresh group, a cryopreservation group, a glutaraldehyde group, and a decellularization plus photo-oxidation group 15 in each group. To evaluate the physical properties of all the rabbit carotid arteries by testing heat-shrinking temperature, tensile stress and the max elongation of each group. Then by buliding subcutaneous embedding model in SD rats we evaluated the biological stability and the anti-calcification function property of the above rabbit carotid arteries, and the detection means included HE stain, atomic absorption spectrometry and Von-Kossa calcium salt stain.
RESULTS:The heat-shrinking temperature, tensile stress and the max elongation in the cryopreservation group were lower or shorter than those of the other groups and the difference had statistical significance (P<0.05). Although the heat-shrinking temperature and the tensile stress in the decellularization plus photo-oxidation group were lower or shorter than those in the glutaraldehyde group (P<0.05), the max elongation in the decellularization plus photo-oxidation group was much longer than that in the glutaraldehyde group (P<0.05). The rabbit carotid artery treated with decellularization plus photo-oxidation showed lower immunogenicity and better biological stability and better anti-calcification property compared with the other groups.
CONCLUSION:Decellularization associated with photo-oxidation is a suitable and novel protocol for small caliber artery allograft with a diameter of less than 6 mm which is unbreakable to mechanical properties and conducive to biological stability, which has a broad prospect.
