Effect of calcitonin gene-related peptide on isoprenaline-induced cardiac fibroblast proliferation and collagen expression.
10.3969/j.issn.1672-7347.2013.06.001
- Author:
Jianzhe LI
1
;
Bin LIU
;
Chenjing WANG
;
Yuanjian LI
;
Jun PENG
Author Information
1. Department of Pharmacology, Central South University, Changsha, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Animals, Newborn;
Calcitonin Gene-Related Peptide;
pharmacology;
Cell Proliferation;
drug effects;
Cells, Cultured;
Collagen Type I;
genetics;
metabolism;
Collagen Type III;
genetics;
metabolism;
Connective Tissue Growth Factor;
genetics;
metabolism;
Fibroblasts;
cytology;
Isoproterenol;
antagonists & inhibitors;
pharmacology;
Myocytes, Cardiac;
cytology;
Primary Cell Culture;
RNA, Messenger;
genetics;
metabolism;
Rats;
Rats, Sprague-Dawley;
Reactive Oxygen Species;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2013;38(6):545-552
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To explore the inhibitory effect of calcitonin gene-related peptide (CGRP) on cardiac fibroblast proliferation and collagen synthesis induced by isoprenaline and the underlying mechanism.
METHODS:The primary cultured cardiac fibroblasts were incubated with isoprenaline (10(-5) mol/L) for 48 h after pretreatment with CGRP (10(-8) or 10(-7) mol/L) for 1 h. Cell activity was detected by MTT. The mRNA expression of collagen (types I and III) and connective tissue growth factor (CTGF) was determined by RT-PCR, and the levels of intracellular ROS were determined by DCFH-DA fluorescent probe.
RESULTS:Isoprenaline significantly promoted fibroblast proliferation and up-regulated collagen (types I and III) and CTGF mRNA expression concomitantly with an increase in ROS production, which were attenuated by CGRP. The effect of CGRP on cardiac fibroblasts was inhibited by CGRP8-37, a selective antagonist of CGRP receptor.
CONCLUSION:CGRP is able to protect cardiac fibroblasts against isoprenaline-induced proliferation and collagen expression, which might be related to the down-regulation of CTGF expression through inhibition of ROS production.
