Effect of NR2E1 on the division and proliferation of neuroblastoma cells.
10.3969/j.issn.1672-7347.2012.06.002
- Author:
Haixia ZHU
1
;
Nan MA
;
Chunhong JIAO
;
Weiwen XUE
;
Zhaojun ZENG
Author Information
1. Department of Neurology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
- Publication Type:Journal Article
- MeSH:
Cell Division;
genetics;
physiology;
Cell Line, Tumor;
Cell Proliferation;
Humans;
Neuroblastoma;
pathology;
RNA, Small Interfering;
genetics;
Receptors, Cytoplasmic and Nuclear;
genetics;
metabolism;
Transfection
- From:
Journal of Central South University(Medical Sciences)
2012;37(6):549-554
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effects of nuclear protein-like transcription factor nuclear receptor subfamily 2 group E member 1 (NR2E1) on the growth, division, and proliferation of neuroblastoma cell line IMR32.
METHODS:A NR2E1 shiRNA plasmid vector was constructed and transfected into neuroblastoma cell line IMR32 using lipofedamine™2000. Subsequent cell growth was measured by cell counting and the protein expression of somatic nuclear division was examined by immunofluorescent staining.
RESULTS:At 48 h after the neuroblastoma cells IMR32 were transfected with NR2E1-shiRNA vector, the related nuclear division protein and the proliferation of the transfected cells IMR32 were remarkably depressed.
CONCLUSION:Cells division and proliferation of neuroblastoma cell line IMR32 is inhibited through transfection with the NR2E1-shiRNA plasmid vector.
