Effect of bone morphogenetic protein-4 on the proliferation and differentiation of rat hepatic precursor cells.
10.3969/j.issn.1672-7347.2011.06.011
- Author:
Juanjuan DONG
1
;
Shan ZENG
;
Miao OUYANG
;
Zenghui HUANG
;
Yuewen GONG
;
Hong SHEN
Author Information
1. Medical Research Center, Xiangya Hospital, Central South University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Bone Morphogenetic Protein 4;
antagonists & inhibitors;
genetics;
physiology;
Carrier Proteins;
pharmacology;
Cell Differentiation;
Cell Line;
Cell Proliferation;
Hepatocytes;
cytology;
Rats;
Recombinant Proteins;
Stem Cells;
cytology
- From:
Journal of Central South University(Medical Sciences)
2011;36(6):539-545
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the regulation effect of bone morphogenetic protein-4 (BMP-4) on the proliferation and differentiation of rat hepatic precursor cells.
METHODS:We used Noggin (200 ng/mL) as the function blocking control of BMP-4, and the hepatic precursor cells of WB-F344 were treated with recombinant BMP-4 at 50 ng/mL at different time points. The proliferation of WB-F344 cells were tested by methyl thiazolyl tetrazolium (MTT) colorimetric assay. The ultrastructural characters of differentiated WB-F344 cells regulated by BMP-4 were observed under a transmission electron microscope. RT-PCR was used to examine mRNA expression of specific molecular markers for different cellular phenotypes potentially differentiated from the WB-F344 cells.
RESULTS:At different time points, the absorbance values in the BMP-4 treatment groups were higher than those in the control groups of Noggin and blank treatment (P<0.01). The WB-F344 cells treated with BMP-4 exhibited typical ultrastructural characters of well-differentiated epithelial cells. The hepatocyte mRNA markers were more significantly promoted in the differentiated WB-F344 cells in the BMP-4 treatment group than those in the other 2 control groups.
CONCLUSION:BMP-4 can promote the proliferation and directional differentiation towards hepatocytes of rat hepatic precursor cells of WB-F344.
