Comparative proteomic analysis of hippocampus between chronic cerebral ischemia rats and normal controls.
10.3969/j.issn.1672-7347.2011.10.010
- Author:
Gang ZHANG
1
;
Fawei WANG
;
Miao GENG
;
Liping CHEN
Author Information
1. Department of Traditional Chinese Medicine, Chinese PLA General Hospital, Beijing 100853, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Brain Ischemia;
metabolism;
Chronic Disease;
Dynamin I;
analysis;
Electrophoresis, Gel, Two-Dimensional;
Female;
Hippocampus;
metabolism;
Male;
Proteome;
analysis;
Proteomics;
methods;
Rats;
Rats, Wistar;
Ubiquitin Thiolesterase;
analysis
- From:
Journal of Central South University(Medical Sciences)
2011;36(10):992-998
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the pathogenesis of brain damage after chronic cerebral ischemia through analysis of the differences in proteins expression in hippocampus between chronic cerebral ischemia rats and normal rats.
METHODS:The chronic cerebral ischemia model was established by ligating the bilateral common carotid arteries.Twenty rats were randomly divided into a model group (n=10)and a sham operation group(n=10). Four weeks later, the differences of proteins expression in hippocampus between model group and sham operation group were analyzed by two dimensional polyacryalmide gel electrophoresis and ultraflex TOF/TOF mass spectrograph.
RESULTS:Compared to the sham operation group, the expressions of 4 proteins were up-regulated and that of 2 proteins were down-regulated in the model group. Six proteins were identified by ultraflex TOF/TOF, which were ubiquitin carboxy-terminal hydrolase L1; Dynamin-1; TMF regulated nuclear protein-like, partial; ATP synthase; rCG50513, isoform CRA_a; and expressed sequence AU016693, isoform CRA_b.
CONCLUSION:Well-resolved and reproducible 2-DE patterns of chronic cerebral ischemia rats were established. Six proteins that correlate with nerve damage after chronic cerebral ischemia are identified.
