ERK signaling pathway mediated epithelial-mesenchymal transition induced by SiO₂ in human bronchial epithelial cells.
10.3969/j.issn.1672-7347.2011.11.009
- Author:
Zhenqin GAO
1
;
Yongbin HU
;
Jingwu PENG
;
Zhenghao DENG
;
Guannan LIANG
;
Haiying JIANG
;
Jianhua ZHOU
Author Information
1. Department of Pathology, Xiangya School of Medicine, Central South University, Changsha 410013, China.
- Publication Type:Journal Article
- MeSH:
Actins;
metabolism;
Bronchi;
cytology;
Cadherins;
metabolism;
Cell Transdifferentiation;
drug effects;
Cells, Cultured;
Epithelial Cells;
cytology;
physiology;
Epithelial-Mesenchymal Transition;
Humans;
MAP Kinase Signaling System;
physiology;
Mitogen-Activated Protein Kinases;
metabolism;
Silicon Dioxide;
pharmacology
- From:
Journal of Central South University(Medical Sciences)
2011;36(11):1085-1089
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the role of extracellular signal regulated kinase (ERK) signaling pathway in SiO₂ induced epithelial-mesenchymal transition (EMT) in human bronchial epithelial cells (HBEC) in vitro.
METHODS:HBEC were treated with SiO₂ (0-300 μg/mL) for 72 h or pretreated with U0126 (0-30 μmol/L) for 1 h and then treated with 200 μg/mL SiO₂ for 72 h. Western blot was used to detect the protein expression of E-cadherin and α-smooth muscle actin (α-SMA). The activity of ERK was examined by mitogen-activated protein kinase (MAPK) activity assay kit in HBEC exposing to SiO₂ (200 μg/mL) for 0-8 h.
RESULTS:The expression of E-cadherin decreased gradually in SiO₂ -stimulated HBEC, and the effect was most significant at 300 μg/mL (P<0.01). The expression of α-SMA increased and the effect was most evident at 200 μg/mL (P<0.01). With SiO₂ treatment, the activity of ERK was upregulated significantly. The phosphorylation of ERK increased at 30 min and decreased after 1 h. U0126 significantly inhibited SiO₂ -induced expression changes in E-cadherin and α-SMA. At 30 μmol/L, the effect was most evident(P<0.01).
CONCLUSION:ERK signaling pathway mediated EMT induced by SiO₂ in HBEC.
