Delayed neuroprotective effect of sevoflurane on the expression of NF-kappaB following transient focal ischemia-reperfusion in rats.
10.3969/j.issn.1672-7347.2010.03.012
- Author:
Zhi YE
1
;
Qulian GUO
;
E WANG
Author Information
1. Department of Anesthesia, Xiangya Hospital, Central South University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Ischemic Attack, Transient;
drug therapy;
metabolism;
Male;
Methyl Ethers;
pharmacology;
therapeutic use;
NF-kappa B;
genetics;
metabolism;
Neuroprotective Agents;
pharmacology;
therapeutic use;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Reperfusion Injury;
metabolism;
prevention & control;
Sevoflurane
- From:
Journal of Central South University(Medical Sciences)
2010;35(3):262-266
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the delayed neuroprotective effect of sevoflurane on the expression of NF-kappaB in rat models of transient focal ischemia-reperfusion.
METHODS:Adult male Sprague-Dawley rats (220 approximately 300 g) were randomly assigned into 3 groups:an ischemia-reperfusion (I/R) group, and a 2.5% Sevoflurane (Sevo1) group, and a 4.0% sevoflurane (Sevo2) group. All the rats were subjected to right middle cerebral artery occlusion (MCAO) for 2 hours. The rats in the I/R group were exposed to pure oxygen 60 min at 24 h before MCAO. Sevoflurane preconditioning was induced 24 h before brain ischemia by exposing the rats to 2.5% or 4.0% sevoflurane + oxygen for 60 min. The effects of sevoflurane on the brain was analyzed by evaluating the infarct volume and the expression of NF-kappaB protein through 2, 3, 5-triphenyltetrazolium chloride (TTC) staining and immunohistochemistry at 24 and 72 h after the reperfusion.
RESULTS:The infarct volumes were significantly reduced in the Sevo1 and Sevo2 groups at 24 and 72 h after the reperfusion, compared with the I/R group. The expression of NF-kappaB in the ischemic territory increased after cerebral ischemia, sevoflurane could remarkably decrease the expression of NF-kappaB in 24 and 72 h after the reperfusion.
CONCLUSION:Sevoflurane inhibits the expression of NF-kappaB protein during focal ischemia the reperfusion,which may be part of the mechanism of its delayed neuroprotective function.
