Effect of formaldehyde on expressions of Fas apoptosis pathway-related genes of ovary tissues in female rats.
10.3969/j.issn.1672-7347.2010.04.010
- Author:
Guoqing PENG
1
;
Caigao ZHONG
;
Qiong ZHANG
;
Ying XIE
;
Fengying GONG
Author Information
1. Department of Toxicology, School of Public Health, Central South University, Changsha 410078, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
drug effects;
genetics;
Caspase 3;
genetics;
metabolism;
Caspase 8;
genetics;
metabolism;
Environmental Pollutants;
toxicity;
Female;
Formaldehyde;
toxicity;
Ovary;
metabolism;
pathology;
RNA, Messenger;
genetics;
metabolism;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
fas Receptor;
genetics;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2010;35(4):341-345
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the mechanism of formaldehyde inducing ovarian toxicity in female rats by observing the effect of formaldehyde on the expression of Fas and caspase-8 mRNA, and the activity of caspase-3 and caspase-8 of ovary tissues in female rats.
METHODS:Forty female Sprague-Dawley(SD) rats were randomly divided into a control group and 3 formaldehyde groups at different concentrations. The rats in the formaldehyde groups were intraperitoneally injected different doses of formaldehyde (20.0,2.0 and 0.2 mg/kg) continuously for 14 days.After 14 days, all rats were sacrificed and their ovaries were collected for detecting the expression of Fas and caspase-8 mRNA with RT-PCR, the protein expression of Fas with Western blot, and the activities of caspase-8 and caspase-3 with spectrophotometric method.
RESULTS:Compared with the control group, the expression of Fas mRNA and its protein and caspase-8 mRNA and the activity of caspase-8 and caspase-3 of ovary tissues in the rats treated with formaldehyde significantly increased with dose (P<0.05).
CONCLUSION:The increase of Fas gene expression and the activity of caspase-8 and caspase-3 may be the important mechanism of ovarian toxicity induced by formaldehyde in female rats.
