Proteomic analysis of morphine rabbit myocardium with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
10.3969/j.issn.1672-7347.2010.06.011
- Author:
Rong TAN
1
;
Dingquan ZOU
;
Yetian CHANG
Author Information
1. Department of Anesthesiology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Electrophoresis, Gel, Two-Dimensional;
Female;
Ischemic Preconditioning, Myocardial;
methods;
Male;
Morphine;
pharmacology;
Myocardial Reperfusion Injury;
metabolism;
Myocardium;
metabolism;
Proteome;
analysis;
Proteomics;
methods;
Rabbits;
Random Allocation;
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization;
methods
- From:
Journal of Central South University(Medical Sciences)
2010;35(6):607-611
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To analyze the morphine rabbit myocardium with matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF-MS).
METHODS:Six New Zealand white rabbits were randomly assigned to a control group (Group C) and a morphine group (Group M). Group C were pretreated with bolus injection of saline 1 mL/kg. Group M were pretreated with bolus injection of morphine 3 mg/kg. The myocardium tissue proteins of the rabbits 24 hours after the injection of morphine or saline preconditioned were extracted and separated by two dimensional gel electrophoresis(2-DE), and the images were analyzed and different proteins were found. Some of the different proteins were determined with MALDI-TOF-MS.
RESULTS:There were 51 protein spots that displayed quantitative changes in expression (P < 0.05), 15 protein spots were chosen for MS analysis, and 8 proteins were preliminarily identified.They were aldose reductase, zinc finger protein 312, src related tyrosine kinase, carbonic anhydrase 12 precursor, electron transfer flavoprotein beta-subunit, glyceraldehyde-3-phosphate dehydrogenase, tumor necrosis factor ligand superfamily member 11 and transmembrane emp24 domain-containing protein.
CONCLUSION:These proteins may be involved in the cardioprotection of morphine preconditioning.
