Differential proteomic analysis of drug resistant A549/DDP cell lines in human lung adenocarcinoma.
10.3969/j.issn.1672-7347.2010.08.013
- Author:
Rui WEI
1
;
Yun XIE
;
Dingyi YANG
;
Lili HE
;
Fang PENG
;
Liangfang SHENG
Author Information
1. Department of Oncology, Xiangya Hospital, Central South University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Adenocarcinoma;
genetics;
metabolism;
pathology;
Annexin A4;
analysis;
Antineoplastic Agents;
pharmacology;
Cell Line, Tumor;
Drug Resistance, Multiple;
genetics;
Drug Resistance, Neoplasm;
genetics;
Electrophoresis, Gel, Two-Dimensional;
HSP27 Heat-Shock Proteins;
analysis;
Humans;
Lung Neoplasms;
genetics;
metabolism;
pathology;
Neoplasm Proteins;
analysis;
classification;
Proteome;
analysis;
Proteomics;
methods
- From:
Journal of Central South University(Medical Sciences)
2010;35(8):854-860
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish 2-dimensional electrophoresis (2-DE) graph of A549 and A549/DDP cell lines, to identify the differentially expressed proteins, and to screen multidrug resistance (MDR) related proteins in human lung adenocarcinoma.
METHODS:The total proteins of A549 and A549/DDP cells were obtained, and were extracted and separated by 2-DE. PDQuest software was applied to analyze the 2-DE images, and the differential proteins of the 2 types of cells were identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS). Western blot was used to determine the expression levels of the 4 proteins.
RESULTS:We established 2-DE maps of total proteins from A549 and A549/DDP. A total of 40 differential protein spots in the 2 cell lines were found, and 23 differential expression proteins were identified by MALDI-TOF-MS. Western blot showed that heat shock protein beta-1, annexin A4, cofilin l, vimentin were differential expression proteins in A549 and A549/DDP, which was consistent with the results of the comparative proteomic analysis.
CONCLUSION:The 23 differential expression proteins in human lung adenocarcinoma are useful for studying the MDR mechanism of lung adenocarcinoma.
