Effect of CpG motif containing oligodeoxynucleotides on dendritic cell function in patients with chronic hepatitis B.
- Author:
Yunhui YOU
1
;
Xuegong FAN
;
Zhenyu HUANG
;
Ning LI
Author Information
1. Department of Rheumatology, Xiangya Hospital, Central South University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Adult;
B7-1 Antigen;
metabolism;
B7-2 Antigen;
metabolism;
Carrier State;
immunology;
Case-Control Studies;
CpG Islands;
Dendritic Cells;
drug effects;
immunology;
Female;
HLA-DR Antigens;
metabolism;
Hepatitis B, Chronic;
immunology;
Humans;
Male;
Oligodeoxyribonucleotides;
pharmacology
- From:
Journal of Central South University(Medical Sciences)
2009;34(6):461-467
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To explore the effect of unmethylated CpG motif containing oligodeoxynucleotides (CpG ODN) on the function of dendritic cells (DCs) in patients with chronic hepatitis B (CHB).
METHODS:DCs were obtained from peripheral blood mononuclear cells (PBMCs) of 15 CHB patients, 12 hepatitis B virus (HBV) carriers, and 10 healthy controls. The expressions of HLA-DR, CD80, and CD86 on DCs were determined by fluorescence activated cell sorting (FACS). The IL-12 level in supernatant of the culture medium was measured by ELISA, and the morphological changes of DCs were observed under transmission electron microscope.
RESULTS:Compared with the controls, DCs stimulated with CpG ODN represented enrichment in cell surface protrusions and rough endoplasmic reticulum, decreased or disappeared vacuole. The expressions of HLA-DR, CD86, and CD80 were much higher in DCs stimulated with CpG ODN than those in complete medium control (P<0.05). When culturing in complete medium, the expressions of HLA-DR, CD86, and CD80 were much lower in CHB patients and HBV carriers than healthy controls (P<0.05). The expressions of HLA-DR and CD86 stimulated with CpG ODN were much lower in CHB patients than HBV carriers and healthy controls (P<0.05). The expressions of CD80 were much lower in CHB patients and HBV carriers than healthy controls (P<0.05). The levels of IL-12 were much higher in DCs stimulated with CpG ODN than that in complete medium controls (P<0.05). The levels of IL-12 in complete medium or medium added with CpG ODN were much lower in CHB patients and HBV carriers than in healthy controls (P<0.05).
CONCLUSION:CpG ODN could significantly promote the maturation of dendritic cells in peripheral blood in CHB patients.
