Expression of NMBR in myometrium in pregnant mice at different gestational ages and its relation with parturition.
- Author:
Weishe ZHANG
1
;
Qingsheng XIE
;
Zhaodi WU
;
Xinhua WU
;
Qinghua LIANG
Author Information
1. Department of Obstetrics and Gynecology, Xiangya Hospital, Central South University, Changsha 410008, China. weishezhang2003@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Animals;
Female;
Gestational Age;
HSP70 Heat-Shock Proteins;
metabolism;
Interleukin-6;
metabolism;
Male;
Mice;
Myometrium;
metabolism;
Parturition;
metabolism;
Pregnancy;
RNA, Messenger;
genetics;
metabolism;
Random Allocation;
Receptors, Bombesin;
genetics;
metabolism;
Signal Transduction;
Transcription Factor RelA;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2009;34(6):531-536
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the spatiotemporal expression of neuromedin B receptor (NMBR) in mice myometrium at different pregnant stages, as well as its mechanism and relation with parturition.
METHODS:The pregnant mice were divided into no-pregnancy (NP), early pregnancy (EP), mid-pregnancy (MP), late-pregnancy (LP), parturition (PT) and postpartum (PP) groups (12 mice in each group), according to pregnant stage. The mRNA and protein expression of NMBR, HSP70 and IL-6 were detected in myometrium in pregnant mice by semi-quantitative RT-PCR and Western blot, while NF-kappaB-P65 DNA binding activity was determined by NoShift transcription factor assay kits, respectively. Their relation with parturition was analyzed.
RESULTS:The mRNA expression level of NMBR in the PT group was significantly higher than that in the NP, EP, LP and PP groups (P<0.05), but this difference was not observed in the MP group (P>0.05). The NMBR protein in PT group was significantly higher than that in the other 5 groups (P<0.01). NF-kappaB-P65 DNA binding activity at PT group was remarkably higher than that in the NP, LP and PP groups (P<0.05). The expression of IL-6 mRNA was significantly higher than that in the NP, LP and PP groups (P<0.05), its protein expression in PT and LP groups was significantly higher than that in the NP and PP groups (P<0.05). The expression of HSP70 mRNA in the PT group was significantly higher than that in the NP and PP groups (P<0.05), and the protein of HSP70 was significantly up-regulated in PT and PP groups compared with in NP and LP groups (P<0.05). The DNA-binding activity of P65 was positively correlated to the mRNA expression of NMBR and IL-6 (r=0.40, P<0.01; r=0.30, P<0.05), so were positively correlated to DNA-binding activity of P65, mRNA expression of HSP70 and NMBR ( r=0.40, P<0.01; r=0.49, P<0.01). DNA-binding activity of P65 did not correlate with the mRNA expression of HSP70.
CONCLUSION:The mRNA and protein expressions of NMBR reach a peak at the onset of labor. NMBR may play an important role in the parturition via NF-kappaB P65-IL-6 signal transduction pathway. It may also influence the onset of labor by regulating HSP70, but this role does not rely on P65 pathway.
