Expression of hypoxia inducible factor-1alpha and erythropoietin in the hippocampus of aging rats.
- Author:
Haiqin WU
1
;
Huqing WANG
;
Juanjuan SHA
;
Yong LI
;
Ru ZHANG
;
Ning BU
Author Information
1. Department of Neurology, Second Affiliated Hospital, Medical College of Xi'an Jiaotong University, Xi'an, China.
- Publication Type:Journal Article
- MeSH:
Aging;
metabolism;
Animals;
Erythropoietin;
metabolism;
Hippocampus;
metabolism;
Hypoxia-Inducible Factor 1, alpha Subunit;
metabolism;
Male;
Rats;
Rats, Sprague-Dawley
- From:
Journal of Central South University(Medical Sciences)
2009;34(9):856-860
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the expression of hypoxia inducible factor-1alpha (HIF-1alpha) and erythropoietin in the hippocampus of aging rats, and to investigate the role of HIF-1alpha and erythropoietin in the aging of nervous system.
METHODS:The expression of Nissl body, HIF-1alpha, and erythropoietin in the CA1 region of the hippocampus in different months was observed by Nissl staining and immunohistochemical technique.
RESULTS:Nerve cells became bigger and appeared sparse, and the Nissl bodies decreased with age. HIF-1alpha positive cells increased significantly with age in the CA1 region of the hippocampus (P<0.05). The expression of erythropoietin presented a parabola with aging in the CA1 region of the hippocampus. The increase from 3 to 18 months and the reduction from 18 to 30 months of erythropoietin positive cells had statistical significance (both P<0.05).
CONCLUSION:HIF-1alpha and erythropoietin are parallelly incremental before middle age, and are separated after middle age, suggesting decreased activity of HIF-1alpha and recession of protein synthesis function may be the main reasons for decreased expression of erythropoietin in the brain during aging. Strengthened endogenous HIF-1alpha activity and supply of exogenous erythropoietin may delay the aging of the nervous system.
