Effect of yizhi jiannao granule on the behavior and neuron apoptosis in SAMP/8 mice.
- Author:
Pin YANG
1
;
Ke-li DONG
;
Wang-yuan ZENG
Author Information
1. Departnent of Geriatrics, Hospital of Traditional Chinese Medicine, Hangzhou 310007, China. yangpin917@hotmail.com
- Publication Type:Journal Article
- MeSH:
Aging;
drug effects;
pathology;
physiology;
Animals;
Apoptosis;
drug effects;
Behavior, Animal;
drug effects;
Drugs, Chinese Herbal;
pharmacology;
Hippocampus;
pathology;
Learning;
drug effects;
Memory;
drug effects;
Mice;
Neurons;
pathology;
Proto-Oncogene Proteins c-bcl-2;
biosynthesis;
genetics;
RNA, Messenger;
biosynthesis;
genetics;
Random Allocation;
bcl-2-Associated X Protein;
biosynthesis;
genetics
- From:
Journal of Central South University(Medical Sciences)
2006;31(1):56-59
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of yizhi jiannao granule concentration fluid (YCF) on the behavior, the apoptosis rate of hippocampus neuron and the expression of apoptosis gene Bcl-2, Bax in senescence accelerated mice Senile-Prone/8 (SAMP/8), and to discuss some mechanism of traditional chinese medicine YCF in improving the capability of learning and memory.
METHODS:Forty 6-month old SAMP/8 mice were randomly divided into the old group, huperzine A (Hup-A) group and YCF group. Ten 4-month old SAMP/8 mice were served as a young control group. Four groups were given different drugs for 8 weeks, their behavior changes were observed, and the hippocampus were taken out to examine the apotosis rate by flow cytomeutry (FCM) and the expression of Bcl-2, Bax mRNA by RT-PCR.
RESULTS:In the YCF group, the escape latency was significantly shortened, the time of swim in the platform quadrant significantly increased, the apoptosis rate of hippocampus neural decreased; the level of Bcl-2 mRNA and the rate of Bcl-2/Bax increased, and the level of Bax mRNA decreased.
CONCLUSION:Yizhi jiannao granule can decrease the neuron apoptosis rate and the Bax level, increase the Bcl-2 level, and modulate the rate of Bcl-2/Bax in SAMP/8 brain, which is probably part of the mechanisms of inhibiting the apoptosis and improving learning and memory.
