Treatment with total hepatic vascular exclusion and reperfusion for the intestinal barrier in rats.
- Author:
De-quan CAO
1
;
Yan-ping CHEN
;
Yong-guo LI
;
Ye-tian CHANG
Author Information
1. Department of Anesthesiology, Second Xiangya Hospital, Central South University, Changsha 410011, China. caodequan989@hotmail.com
- Publication Type:Journal Article
- MeSH:
Animals;
Bacterial Translocation;
Female;
Intestinal Mucosa;
microbiology;
pathology;
Ischemia;
pathology;
physiopathology;
Liver;
blood supply;
Male;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Reperfusion Injury;
pathology;
physiopathology
- From:
Journal of Central South University(Medical Sciences)
2005;30(4):433-436
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the influence of treatment with total hepatic vascular exclusion and reperfusion on the intestinal barrier in rats.
METHODS:The total hepatic vascular exclusion and reperfusion model was built after the block of hepatic portal, suprahepatic and infraheptic vena cava for 20 minutes. Sixty Sprague-Dawley rats were divided randomly into 2 groups: sham operation group (Group A, n=30) and total hepatic vascular exclusion and reperfusion treatment group (Group B, n=30). Each group was subdivided randomly into 3 subgroups (n=10) according to different experiment time points as follows: at the end of the total hepatic vascular exclusion (T0), 4 reperfusion after total hepatic vascular exclusion (T1) and the 48 h survival. Portal vein blood gas was analysed at T0. At T0 and T1 the following items were detected: the level of portal vein blood D-lactate, tumor necrosis factor-alpha (TNF-alpha), the MDA concentration and pathologic morphology change of intestinal mucosa.
RESULTS:Compared with Group A, the PCO2 at T0 in Group B increased while pH, P02, and HCO3- decreased significantly (P < 0.05). The level of portal blood D-lactate, TNF-alpha and intestinal mucosa MDA at T0 and T1 was significantly higher (P < 0.05, or P < 0.01). The histologic damage in the intestinal mucosa was observed in Group B, and the rat survival in Group B was lower than that in Group A (P < 0.05).
CONCLUSION:The treatment with total hepatic vascular exclusion and reperfusion can damage the intestinal barrier in rats.
