Microvessel counts and the expressions of chemotactic factors in the pathological scar tissues.
- Author:
Li QIAN
1
,
2
;
Bai-Cheng ZHAO
;
Li PI
;
Qing LU
Author Information
1. Department of Burn and Plastic Surgery, Second Xiangya Hospital, Central South University, Changsha 410011, China. HNFIJ@
2. com
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Burns;
complications;
Capillaries;
metabolism;
Chemokine CCL2;
biosynthesis;
genetics;
Chemokine CCL3;
Chemokine CCL4;
Cicatrix;
etiology;
metabolism;
Female;
Humans;
Interleukin-8;
biosynthesis;
genetics;
Macrophage Inflammatory Proteins;
biosynthesis;
genetics;
Male;
Middle Aged;
RNA, Messenger;
biosynthesis;
genetics;
Skin;
blood supply
- From:
Journal of Central South University(Medical Sciences)
2005;30(3):340-348
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the microvessel counts and the expressions of interleukin-8 (IL-8), monocyte chemoattractant protein-1 (MCP-1), and macrophage inflammatory protein-1 ( MIP-1) alpha mRNA in the pathological scar tissues.
METHODS:Immunohistochemical method of avidin-biotin complex was used for microvessel counts on the routinely formalin-fixed and paraffin-embedded sections of specimens of hypertrophic scars, keloids, normal skin, and surgical scar, and in situ hybridization for the expressions of IL-8, MCP-1, MIP-1alpha mRNA.
RESULTS:The microvessel counts as well as the positive rates and the scorings of IL-8, MCP-1, and MIP-1alpha mRNA were significantly higher in pathological scars than those in the normal skin and surgical scar (all P < 0.05). The microvessel counts were significantly higher in the positive cases of IL-8, MCP-1 and MIP-1alpha mRNA than those in the negative ones (P < 0.05). The close positive correlations were found among the microvessel counts and the expressive scorings of 3 factors (P < 0.05). The close positive correlations were also found among the expressive scorings of IL-8, MCP-1, and MIP-1alpha mRNA in pathological scars. Microvessel counts were significantly higher in hypertrophic scars with the course less than 1 year than those with the course more than 1 year.
CONCLUSION:IL-8, MCP-1 and MIP-1alpha play important roles in promoting the neovascularization of pathological scars.
