Expression of PROK 1 and its receptor PROKR 1 in endometriosis and its clinical significance.
10.11817/j.issn.1672-7347.2019.06.003
- Author:
Yahong WU
1
;
Xianqing WU
1
Author Information
1. Department of Obstetrics and Gynecology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
- Publication Type:Journal Article
- MeSH:
Endometriosis;
Endometrium;
Female;
Gastrointestinal Hormones;
metabolism;
Humans;
RNA, Messenger;
Receptors, G-Protein-Coupled;
metabolism;
Vascular Endothelial Growth Factor, Endocrine-Gland-Derived;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2019;44(6):621-627
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the role of prokineticin (PROK) 1 and prokineticin-receptor (PROKR) 1 in the pathogenesis of endometriosis and its clinical signifaicance.
Methods: Quantitative real-time PCR (qPCR) and Western bloting were used to detect the expression of PROK 1 and PROKR 1 in eutopic and ectopic endometrium of endometriosis (n=22) and normal control endometrium (n=18). Endometrial stromal cells were isolated and cultured in 6 normal controls. The expression of PROK 1 mRNA was detected by qPCR after treated with estradiol (E2) or TNF-α.
Results: PROK 1 and PROKR 1 mRNA were expressed in eutopic and ectopic endometrium of endometriosis and normal control endometrium, and the expression level gradually declined (P<0.05). The expression of PROKR-1 protein in eutopic and ectopic endometrium of endometriosis and normal control endometrium gradually declined (P<0.05). The expression of PROK-1 protein in normal control endometrial cells and eutopic endometrium cell was higher in secretory phase than in proliferative phase (P<0. 05). E2 did not change the expression of PROK 1, whereas TNF-α up-regulated the expression of PROK 1.
Conclusion: PROK-1 and its receptors are involved in the pathogenesis and development of endometriosis. TNF-α can promote angiogenesis via up-regulating the expression of PROK 1.
