Inhibition of lipopolysaccharide-induced inflammation in RAW264.7 macrophages by sinomenine through regulating heme oxygenase-1 expression and autophagy.
10.11817/j.issn.1672-7347.2018.09.006
- Author:
Yue PENG
1
;
Hao OU
1
;
Mingshi YANG
1
;
Yu JIANG
2
;
Min GAO
1
Author Information
1. Department of Critical Care Medicine, Third Xiangya Hospital, Central South University, Changsha 410013, China.
2. Institute of Emergency Medicine; Hunan Provincial Key Laboratory of Emergency and Critical Care Metabonomics, Hunan Provincial People's Hospital, Changsha 410005, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Anti-Inflammatory Agents;
pharmacology;
Autophagy;
drug effects;
Gene Expression Regulation, Enzymologic;
drug effects;
Heme Oxygenase-1;
genetics;
Inflammation;
chemically induced;
Lipopolysaccharides;
Macrophages;
drug effects;
Mice;
Morphinans;
pharmacology
- From:
Journal of Central South University(Medical Sciences)
2018;43(9):964-970
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effect of sinomenine on lipopolysaccharide (LPS)-induced inflammation in RAW264.7 macrophages and the underlying mechanisms.
Methods: The mouse RAW264.7 macrophages were treated with sinomenine and/or LPS with or without heme oxygenase-1 (HO-1) inhibitor Znpp. Real-time PCR, ELISA, immunofluenscence, and Western blot were used to detect the mRNA expression of TNF-α and IL-6, the release of TNF-α and IL-6, the protein expression of HO-1 and autophagy, respectively.
Results: Compared with the control group, the mRNA expression and release of inflammatory cytokines TNF-α and IL-6 were increased, the green fluorescence of autophagy-related protein LC3 was accumulated and the protein expression of HO-1 was increased in RAW264.7 cells after LPS treatment (P<0.05). Compared with the LPS group, sinomenine treatment could reduce the mRNA expression and release of TNF-α and IL-6, accompanied by increasess in green fluorescence aggregation of LC3 and HO-1 production (P<0.05). HO-1 inhibitor Znpp could weaken the ability of sinomenine through suppressing TNF-α and IL-6 expression and decreasing the aggregation of LC3 green fluorescence (P<0.05).
Conclusion: Sinomenine could alleviate LPS-induced inflammation in RAW264.7 macrophages, which might be related to HO-1 mediated autophagy. This study provides an experimental and theoretical basis for the clinical application of sinomenine in prevention and treatment of inflammation.
