Effect of umbilical cord mesenchymal stem cells on proliferation and apoptosis of ovarian cancer cells.
10.11817/j.issn.1672-7347.2019.180794
- Author:
Lina ZHOU
1
;
Jiangdong XIANG
1
;
Linxia LI
1
;
Wei CHEN
1
;
Xiaowei XI
1
Author Information
1. Department of Obstetrics and Gynaecology, Shanghai General Hospital of Nanjing Medical University, Shanghai 200080, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Cell Line, Tumor;
Cell Proliferation;
Female;
Humans;
Mesenchymal Stem Cells;
Ovarian Neoplasms;
Umbilical Cord
- From:
Journal of Central South University(Medical Sciences)
2019;44(10):1120-1127
- CountryChina
- Language:Chinese
-
Abstract:
To observe the effects of human umbilical cord mesenchymal stem cells (UC-MSCs) on the proliferation and apoptosis of human ovarian cancer cell SKOV3.
Methods: Transwell co-culture was used to observe the targeted homing effect of UC-MSCs on ovarian cancer cells. MTT assay was used to detect the inhibitory effect of UC-MSCs conditioned medium on SKOV3 proliferation, and Annexin V-FITC/PI double staining was used to detect the apoptotic rate. Real-time PCR was used to detect the expression levels of Ki-67, Bcl-2 and Bax genes-relevant to proliferation and apoptosis of SKOV3 cells.
Results: UC-MSCs targeted SKOV3 cells in vitro. MTT assay showed that UC-MSCs conditioned medium significantly inhibited the proliferation of SKOV3 cells (P<0.01). Annexin V-FITC/PI double staining showed that the apoptotic rate in the 75% conditioned medium group was significantly higher than that in the control group (P<0.05). Real-time PCR showed that the expression of proliferation-related gene Ki-67 decreased significantly (P<0.01). The apoptosis-related gene Bcl-2 expression was decreased dramatically (P<0.01), and Bax expression was increased significantly (P<0.01).
Conclusion: UC-MSCs can target ovarian cancer cells in vitro, inhibit the proliferation of SKOV3 cells by regulating the expression of Ki-67, and promote the apoptosis of SKOV3 cells by regulating the expression of Bcl-2 and Bax.
