Effect of silencing the VDR gene on the migration and invasion of prostate cancer cells.
- Author:
Yuan-Dong ZHANG
1
;
Hui ZHAO
1
;
Kang-Jian LI
1
;
Run-Yun GUAN
1
Author Information
1. Department of Urology, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, China.
- Publication Type:Journal Article
- Keywords:
gene interference;
invasiveness;
shRNA;
vitamin D receptor;
PC-3 cell
- MeSH:
Cell Line, Tumor;
Cell Movement;
genetics;
Cell Proliferation;
Down-Regulation;
Gene Silencing;
Humans;
Lentivirus;
Male;
Neoplasm Invasiveness;
genetics;
Plasmids;
Prostatic Neoplasms;
genetics;
pathology;
RNA, Messenger;
metabolism;
RNA, Small Interfering;
Receptors, Calcitriol;
genetics;
metabolism;
Transfection;
Wound Healing;
genetics
- From:
National Journal of Andrology
2017;23(11):969-974
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of small interfering RNA silencing the vitamin D receptor (VDR) on the biological behavior of prostate cancer PC-3 cells.
METHODS:We constructed the VDR-shRNA lentiviral vector and determined the mRNA and protein expressions of VDR by RT-PCR and Western blot. Using scratch wound healing and Transwell chamber assays, we detected the changes in the migration and invasiveness of the PC-3 cells after silencing VDR.
RESULTS:The VDR-shRNA plasmid significantly interfered the VDR expression and successfully screened the cell lines with stable VDR-shRNA interference. The rate of scratch wound healing was markedly lower in the VDR interference group than in the blank control and LV3 negative control groups (59% vs 73.6% and 77.8%, P <0.05), but with no statistically significant difference between the latter two (P >0.05), and so was the count of permeable cells (P <0.05), but with no significant difference between the latter two groups, either (P >0.05). The migration ability and invasiveness of the VDR-treated cells were remarkably decreased as compared with those of the control cells.
CONCLUSIONS:Down-regulated expression of the VDR gene may reduce the migration and invasiveness of prostate cancer cells.
