Anti-proliferative and apoptotic effects of S1, a tetrandrine derivative, in human gastric cancer BGC-823 cells.
10.1016/S1875-5364(16)30062-0
- Author:
Rong-Rong LEI
1
;
Hai-Feng HU
2
;
Fan BAI
3
;
Ying LIU
2
;
Chun-Zhen WU
4
,
5
;
Xiao-Xing HUANG
1
;
Li-Ping XIE
1
;
You-Jia HU
1
Author Information
1. Shanghai Institute of Pharmaceutical Industry, Shanghai 200040, China.
2. Shanghai Institute of Pharmaceutical Industry, Shanghai 200040, China; Sinopharm Health Industry Research Co., Ltd., Shanghai 200040, China.
3. The Kochi Prefectural Makino Botanical Garden, Kochi 4200-6, Japan.
4. Shanghai Institute of Pharmaceutical Industry, Shanghai 200040, China; Sinopharm Health Industry Research Co., Ltd., Shanghai 200040, China. Electronic address: czw1962@
5. com.
- Publication Type:Journal Article
- Keywords:
Apoptosis;
BGC-823 cells;
G2/M phase arrest;
Tetrandrine derivative
- MeSH:
Antineoplastic Agents;
pharmacology;
Apoptosis;
drug effects;
Benzylisoquinolines;
chemistry;
pharmacology;
Caspase 3;
genetics;
metabolism;
Caspase 9;
genetics;
metabolism;
Cell Cycle Checkpoints;
drug effects;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Cell Survival;
drug effects;
Humans;
Proto-Oncogene Proteins c-bcl-2;
genetics;
metabolism;
Stomach Neoplasms;
drug therapy;
enzymology;
genetics;
physiopathology;
bcl-2-Associated X Protein;
genetics;
metabolism
- From:
Chinese Journal of Natural Medicines (English Ed.)
2016;14(7):527-533
- CountryChina
- Language:English
-
Abstract:
The aim of the study was to investigate the anti-proliferation and apoptosis-inducing effects of S1, a novel tetrandrine derivative, in human gastric cancer BGC-823 cells and explore the possible mechanism of action. The anti-proliferative activity was determined by MTT assay; the induction of cell cycle arrest and apoptosis were detected by flow cytometry. Quantitative real time RT-PCR and Western blotting were used to evaluate the mRNA and protein expression levels in mitochondrial pathway. S1 significantly reduced cell viability and induced a G2/M phase arrest and apoptosis in dose- and time-dependent manner. Further studies showed that S1 increased mRNA and protein expression of Bax and the Bax/Bcl-2 ratio. Moreover, S1 decreased the protein expression of procaspase-9 and procaspase-3, suggesting that the induction of apoptosis may be related to the alteration of the ratio of Bax/Bcl-2 and the activation of caspases. These findings suggested that S1 merits further investigation as a novel therapeutic agent for the treatment of human gastric cancer.
