Six new cytotoxic and anti-inflammatory 11, 20-epoxy-ent-kaurane diterpenoids from Isodon wikstroemioides.
10.1016/S1875-5364(15)30030-3
- Author:
Hai-Yan WU
1
;
Wei-Guang WANG
2
;
Xue DU
2
;
Jin YANG
1
;
Jian-Xin PU
3
;
Han-Dong SUN
4
Author Information
1. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; University of Chinese Academy of Sciences, Beijing 100049, China.
2. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
3. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. Electronic address: pujianxin@mail.kib.ac.cn.
4. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. Electronic address: hdsun@mail.kib.ac.cn.
- Publication Type:Journal Article
- Keywords:
Anti-inflammatory activity;
Cytotoxicity;
Isodon wikstroemioides;
Isowikstroemins H-M
- MeSH:
Anti-Inflammatory Agents;
isolation & purification;
Antineoplastic Agents, Phytogenic;
isolation & purification;
Cell Line, Tumor;
Diterpenes, Kaurane;
isolation & purification;
Humans;
Inhibitory Concentration 50;
Isodon;
chemistry;
Lipopolysaccharides;
pharmacology;
Macrophages;
drug effects;
metabolism;
Neoplasms;
drug therapy;
Nitric Oxide;
antagonists & inhibitors;
biosynthesis;
Phytotherapy;
Plant Components, Aerial;
Plant Extracts;
isolation & purification
- From:
Chinese Journal of Natural Medicines (English Ed.)
2015;13(5):383-389
- CountryChina
- Language:English
-
Abstract:
The present study was designed to determine the chemical constituents of EtOAc extracts of the aerial parts of Isodon wikstroemioides. Compounds 1-8 were isolated and purified by normal-phase silica gel and reversed-phase C18silica gel column chromatography and HPLC. Their structures were elucidated by extensive spectroscopic methods. Most of them were evaluated for their in vitro cytotoxicity against human cancer HL-60, SMMC-7721, A-549, MCF-7, and SW-480 cells and their inhibitory activity against nitric oxide (NO) production in LPS-activated RAW264.7 macrophages. Among the eight 11, 20-epoxy-ent-kauranoids isolated, compounds 1-6 (isowikstroemins H-M) were new diterpenoids. Compounds 1, 3, and 7 exhibited significant cytotoxicity with IC50 values ranging from (0.84 ± 0.02) to (4.09 ± 0.34) μmol · L(-1), while compounds 4 and 5 showed selective cytotoxicity. In addition, compounds 1, 3, 4, and 7 exhibited inhibitory activity against nitric oxide (NO) production in LPS-activated RAW264.7 macrophages. These results provide a basis for future development of these compounds as anti-cancer and anti-inflammatory agents.
