Effect of type 2 diabetes mellitus on flavonoid pharmacokinetics and tissue distribution after oral administration of Radix Scutellaria extract in rats.

Xue-Ju ZHANG; Shu LIU; Jun-Peng XING; Zhi-Qiang LIU; Feng-Rui SONG

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Effect of type 2 diabetes mellitus on flavonoid pharmacokinetics and tissue distribution after oral administration of Radix Scutellaria extract in rats.

Author: Xue-Ju ZHANG ¹ ; Shu LIU ² ; Jun-Peng XING ² ; Zhi-Qiang LIU ³ ; Feng-Rui SONG ⁴
Author Information

1. National Center of Mass Spectrometry in Changchun, Jilin Province Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China; University of Chinese Academy of Sciences, Beijing 100039, China.
2. National Center of Mass Spectrometry in Changchun, Jilin Province Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
3. National Center of Mass Spectrometry in Changchun, Jilin Province Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China. Electronic address: liuzq@ciac.ac.cn.
4. National Center of Mass Spectrometry in Changchun, Jilin Province Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China. Electronic address: songfr@ciac.ac.cn.
Publication Type:Journal Article
Keywords: Diabetes mellitus; Flavonoid; Pharmacokinetics; Radix Scutellaria; Tissue distribution
MeSH: Administration, Oral; Animals; Chromatography, High Pressure Liquid; Diabetes Mellitus, Experimental; drug therapy; metabolism; physiopathology; Drugs, Chinese Herbal; administration & dosage; chemistry; Flavonoids; analysis; chemistry; pharmacokinetics; Male; Molecular Structure; Plant Roots; chemistry; Rats; Rats, Wistar; Reproducibility of Results; Scutellaria baicalensis; chemistry; Tandem Mass Spectrometry; Tissue Distribution; physiology
From: Chinese Journal of Natural Medicines (English Ed.) 2018;16(6):418-427
CountryChina
Language:English
Abstract: Radix Scutellaria is widely applied to the treatment of diabetes mellitus in China. Its main bioactive constituents contain baicalin, wogonoside, oroxyloside, and their aglycones. To investigate the effect of type 2 diabetes mellitus on both pharmacokinetics and tissue distribution of these flavonoid compounds, the six flavonoids in plasma and tissues from the normal and type 2 diabetic rats after oral administration of Radix Scutellaria extract were simultaneously measured by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method. The results showed that baicalin, wogonoside, and oroxyloside had higher C and AUC values (P < 0.05) in type 2 diabetic rats than that in normal rats and the tissue-distribution behaviors of the six flavonoid compounds in hearts, livers, spleens, lungs, kidneys, brains, pancreas, fat and muscle of the type 2 diabetic rats showed obviously differences from the normal rats (P < 0.05). In conclusion, the differences in the pharmacokinetics of oroxyloside and tissue distribution of the six flavanoids in Radix Scutellaria extract between diabetic and normal rats were found for the first time. The results from the present study provided a crucial basis for a better understanding of in vivo anti-diabetic mechanism of action of the six flavonoids from Radix Scutellaria.